Home > Search Results: GPP503/12/P395
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study
 Benkovská, Dagmar ; Flodrová, Dana ; Bobálová, Janette ; Laštovičková, Markéta
N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies.
Detailed record
Monitoring of quantitative and qualitative barley proteome changes within different varieties and malting process
 Flodrová, Dana ; Benkovská, Dagmar ; Bobálová, Janette
Various European barley cultivars recommended as the varieties suitable for the production of the Czech beer were analyzed in this work. Relative quantification of chosen protein and their comparison was evaluated using the combination of isotopic peptides labeling (iTRAQ) and mass spectrometry. Barley grain proteins were separated by HPLC on the SEC-3 column and chosen identified protein fractions were subsequently used for iTRAQ labeling.
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English