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Testing of nanofibers as potential sorbents for solid phase extraction using sequential injection analysis
 Jirsíková, Veronika ; Horstkotte Šrámková, Ivana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Veronika Jirsíková Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Consultant: Assoc. Prof. PharmDr. Hana Sklenářová, Ph.D. Title of diploma thesis: Testing of nanofibers as potential sorbents for solid phase extraction using sequential injection analysis This diploma thesis deals with the testing of various types of nanofibers which can be used as sorbents in solid phase extraction in a sequential injection analysis system. The tested nanofibers were made by electrospinning. The polyamide (PA) and polyvinylidene fluoride (PVDF) nanofibers were used. Female sex hormones, non-steroidal anti-inflammatory drugs, H2 antihistamines, bisphenols and clenbuterol were chosen as model analytes. The analysis was carried out in the sequentional injection analysis (SIA) in which a polymethylmethacrylate column was integrated. The column was filled with one type of the tested nanofibers. The detection was performed by a spectrophotometer at a wavelength of absorption maximum of the selected analytes. The retention of individual analytes on the nanofibers was observed depending on the chemical structure and the physicochemical properties. The most measurements were carried out with polyvinylidene fluoride...
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