Rigorous theses

Rigorous theses 14,313 records found  previous11 - 20nextend  jump to record: Search took 0.01 seconds. 
Substantiation of electronic evidence
Jirounková, Kristýna ; Gřivna, Tomáš (advisor) ; Mulák, Jiří (referee)
Substantiation of Electronic Evidence Abstract The author focuses on the possibility of "misuse" of data as electronic evidence in the thesis. She describes specifics of electronic evidence and she points out that current technologies are so advanced that they are able to track and map the lives of individuals. The author simulates the investigation of the crime of murder, based on the idea, that thanks to the daily stored data on digital activity of persons in conjunction with GPS data, the physical activity of persons can be proven. She seeks to investigate this crime regardless of the regulation of fundamental human rights and freedoms only by electronic evidence. Eventually she assesses the procedure in the light of legislation. Finally, the author deals with the issue of confidentiality obligations of attorneys at law in connection with the use of electronic devices for the performance of their activities and she points out specifics of search of premises, where attorneys at law perform advocacy. Kristýna Jirounková Thesis 2021 Charles University, Faculty of Law
Plasmid constuction for expression of UDP-glucosyltransferase from Haemonchus contortus
Machalová, Barbora ; Matoušková, Petra (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Barbora Wengrynová Supervisor: doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Plasmid construction for expression of UDP-glukosyltransferase from Haemonchus contortus The barber's pole worm (Haemonchus contortus), like other organisms, uses biotransformation enzymes to metabolize exogenous and endogenous substances. Important representatives of these enzymes are also UDP-glycosyltransferases, which catalyze the formation of a covalent bond between hexose activated by uridine diphosphate and the metabolized compound, thus helping to eliminate undesirable substances. Increased attention to these enzymes is due to the involvement of UDP-glycosyltransferases in the development of resistance to anthelmintics, which are used to treat hemonchosis. The study of their properties and functions can significantly help in solving resistance issues. UGTs are divided into several families and subfamilies depending on sequence similarities. This work focuses on three representatives of UGT in the Haemonchus contortus, UGT24C1, UGT365B6 a UGT368B2. The theoretical part of the diploma thesis includes knowledge about the Haemonchus contortus, hemonchosis and its treatment, biotransformation enzymes and...
Specifics of education and activation of seniors in residential facilities
Janků, Vítězslav
The thesis deals with the issue of education and activation of seniors in residential facilities - homes for the elderly. In the theoretical part it deals with the quality of life of seniors, motivation for further education, intergenerational learning, the topic of aging and old age in the context of demographic, socio-political, health, cultural and legislative aspects. It is the demographic development of society that is a major impetus for change in the field of professional institutional care for the elderly. Specific integrative, counselling, therapeutic and rehabilitation approaches and conceptualisations of these topics are set within the framework of andragogy and gerontagogy. The research focuses on two target groups: on the one hand, the elderly themselves who have completed educational and activation programmes and on the professionals who implement or prepare (methodologically, managerially) these activities and activities. The aim of the thesis is to observe the interdisciplinary importance of education, animation and activation for the development of cognitive abilities of individuals and their quality of life. The research assumption is that active and motivated individuals are more satisfied and do not depend on the help of their environment. Their self-sufficiency is therefore...
Loyalty and dedication of employees in pharmacy in relation to evaluation and remuneration
Bittner, Josef
(AJ): The work entitled "Loyalty and dedication of pharmacy staff in relation to evaluation and remuneration" deals with the issue of maintaining the satisfaction and loyalty of employees in pharmacy, specifically in pharmacy networks. It focuses on finding the relationship between satisfaction, loyalty and the form of management and remuneration. The qualitative questionnaire survey helps to find those aspects of maintaining loyalty and satisfaction that can be developed and strengthened by strategic management in such a professional sector.
Implementation of digital technologies in education
Přibyl Durlinová, Dominika
The aim of this thesis is to analyze strategic and other documents and data that are focused on distance learning in primary schools. The aim is also to describe how distance teaching took place in primary schools using digital technologies and to point out its specifics, including positives and negatives. Strategic and other documents are the basis for determining the readiness of teachers and schools for the transition to distance learning. Due to the COVID-19 pandemic, schools were closed and it was necessary to move to distance learning, which was online. Schools and educators had to adapt and implement digital technologies in education in a short period of time. The diploma thesis focuses on the implementation of digital technologies in education in distance learning. In the modern world, every person is increasingly dependent on the use of technology which is not the case in education. Teachers are key players in implementing digital technologies in education. The implementation of digital technologies in education must be seen not only as a technological process, but also as a pedagogical process. This work is a qualitative analysis that deals with digital technologies during distance learning. The theoretical part is developed on the basis of a search of professional literature focused on...
Theories of Judicial Decision-Making confronted the Functioning of Czech Courts
Blažková, Kristina ; Tryzna, Jan (referee)
Theories of Judicial Decision-Making confronted the Functioning of Czech Courts Abstract Judicial decision-making in hard cases is not a rationalisation which legitimates a choice made based on non-legal grounds, but a relatively predictable, determined and just process which draws legitimacy from the rationality of legal thinking and is significantly influenced by the judge's effort to make the best decision possible considering his conception of law and general training. Based on this proposition the dissertation thesis explores the effect of the judge's conception of law and his judicial function on his legal argumentation and his decision-making in hard cases. The main concept the thesis thus being the judicial philosophy. The dissertation thesis firstly analyses the concept theoretically and subsequently tests the theory on real judicial practice. The hypothesis is that judges of apex courts have different judicial philosophies and that their opposing views on grounds of law and their judicial function exhibits itself in their decision-making in hard cases. The disagreement between judges may be characterised as reasonable disagreement. In situations of reasonable disagreement, the opposing parties are incapable of reaching a compromise despite perfectly sound and grounded positions and mutual effort...
HPLC analysis of podophyllotoxin
Nováková, Markéta ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Markéta Nováková Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: HPLC Analysis of Podophyllotoxin The diploma thesis is focused on optimalization of HPLC method for the analysis of podophyllotoxin. The method was developed for the analysis of Juniperus virginiana leaves extract. Various stationary phases, mobile phases, temperatures, ultrasonic extraction times were tested. Following conditions were chosen as optimal: 30 minutes ultrasonic extraction time, LiChrospher 100 RP-18 250-4 column, 0,1% CH3COOH:ACN 45:55 mobile phase, temperature 40 řC, flow rate 0,8 ml/min, sample volume 3 μl, fluorescence detection λex 240 nm, λem 320 nm, time of analysis 9 minutes. The method was also developed for the analysis of plant tissue cultures of Juniperus virginiana, for which gradient elution was chosen. This method had following conditions: LiChrospher 100 RP-18 250-4 column, mobile phase 0,1% CH3COOH:ACN, change of acetic acid ratio: 0-8 min 70-10 %, 8-9 min 10 %, 9-10 min 10- 70 %, 10-12 min 70 %, temperature 40 řC, flow rate 0,8 ml/min, sample volume 3 μl, fluorescence detection λex 240 nm, λem 320 nm, time of analysis 12 minutes. Subsequently, both methods were...
Testing of potential capability of chiral ionic liquids with long alkyl chain in capillary electrophoresis chiral separations II
Müllerová, Kateřina ; Jáč, Pavel (advisor) ; Lochman, Lukáš (referee)
Department: Department of Analytical Chemistry Candidate: Kateřina Műllerová Supervisor: PharmDr. Pavel Jáč, Ph.D. Title of the diploma thesis: Testing of potential capability of chiral ionic liquids with long alkyl chain in capillary electrophoresis chiral separations II Chiral ionic liquid (−)-N-dodecyl-N-methylephedrinium bromide (DMEB) was tested as a selector for chiral separations of selected drugs such as chiral quinolones, ketoprofen, and flurbiprofen by capillary electrophoresis (CE). The effect of several parameters on enantioseparation was examined: (i) type and pH of the separation buffer, (ii) type and amount of organic modifier, and (iii) the concentration of DMEB. When using this chiral selector, only the separation of ofloxacin enantiomers was observed, while the enantiorecognition of other chiral model analytes was not successful under the conditions tested. As a next step, a CE method for the assay of levofloxacin was developed to demonstrate the potential of DMEB as a chiral selector in quality control of single-isomer drugs. The best separation was achieved with 20 mM tris buffer of pH 8.5 containing 100 mM DMEB and 20 % (v/v) of acetonitrile as the background electrolyte. The separation took place in 50 µm id fused silica capillary (80.5 cm / 72 cm) at −30 kV with UV detection...
Effect of selected pathological states on mitochondrial function in mammalian cells and tissuesMitochondrial dysfunction in sepsis
Kohoutová, Michaela
Sepsis, or life-threatening organ dysfunction caused by deregulated host response to the presence of infection, is one of the most significant causes of death caused by infection diseases worldwide. 31.5 million patients get sick with sepsis annually and 5.3 million of them die. Sepsis is characterised by homeostatic dysbalance, which can lead to septic shock or even to death. Sepsis may also result in development of multiorgan dysfunction or damage to several organs at the same time. Especially heart, kidneys, liver, lungs, central nervous system and blood elements belong among the organs affected by sepsis. One of the most affected organs is the heart, impairment of which is accompanied with the development of myocardial depression, which contributes to the increase of mortality from sepsis. The precise mechanisms leading to septic myocardial depression have not been fully explained yet, nevertheless mitochondria appear to play an important role in this process. Due to sepsis, many mitochondrial functions are disrupted, which is subsequently manifested by the depletion of cellular energy stores, resulting in myocardial depression. In our laboratories, we managed to create a clinically relevant model of sepsis and septic shock, specifically sepsis caused by fecal peritonitis in domestic pigs, that...
The biology of aging in Nothobranchius fishes
Žák, Jakub
The aging human population and consequent unprecedented demographic changes in society have inevitably led to more scientists focusing their research on aging. Senescence, in the narrowest biological sense, is age related decline in an organism's function and survival. The majority of senescence research is performed in the laboratory environment. The basic biology of wild counterparts of model organisms is therefore relatively unstudied, despite its importance for proper interpretation of laboratory outcomes. This dissertation, via a series of 6 first-authored and 5 second-authored papers, combines field and laboratory research on senescence and senescence-related life history traits using a short-lived fish Nothobranchius furzeri. An age at the maturity of 14 days post hatching in wild N. furzeri is presented in this thesis as well as extremely fast growth of wild fish. Both life history traits have important consequences for senescence. The following section is focused on how the environment modulates senescence. In the first step, preferred body temperatures of Mozambican Nothobranchius fish were measured and descriptions of environmental and behavioural fluctuations within a 24 hours periodicity were completed. Based on this knowledge, ecologically relevant thermal fluctuations were simulated...

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