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National Repository of Grey Literature

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Cell layer cultivation in the microfluidic system Kachan, Ksenia ; Svoboda, Ondřej (referee) ; Chmelíková, Larisa (advisor) The theoretical part of this thesis describes the principles of cell culture in vitro and modern in vitro models for endothelial cell layer cultivation. It also describes the principles of confocal and fluorescence microscopy. The practical part is dedicated to working with cell cultures and realization of experiment with cell cultivation in microfluidic system. Cell layer was photographed using laser confocal scanning microscope Leica TCS SP8 X during the entire experiment. For processing and analysis of the obtained images, an algorithm in the software MATLAB was proposed. Detailed record

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