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Analysis of TP53 gene transcriptional varians in human leucemic cells
 Vlahová, Veronika ; Mgr.Diana Grochová, Ph.D. (referee) ; Malčíková,, Jitka (advisor)
The theoretical part of the thesis summarizes general information about the human TP53 gene and p53 protein, which is encoded by this gene. There is also dealt with the transcriptional variants - p53 isoforms. The experimental part is focused on the detection of isoform p53 and its shorter, yet undescribed form. The starting material is peripheral blood of patients from University Hospital Brno, from which RNA was isolated. Subsequently, RNA was transcribed by reverse transcription into cDNA which was amplified by polymerase chain reaction (PCR). Experimental measurements demonstrate that p53 occurs in all the B-lymphocytes of patients with chronic lymphocytic leukemia. It also demonstrats the existence of a shorter form of p53.
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