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Studium polymorfizmu DNA mrkve
Sejbalová, Darina
This work is focused on theoretical and practical study of genetic diversity in carrots species. The aim of this work was to analyze the DNA polymorphism in Daucus carota L. by using molecular biology methods based on DNA isolation, PCR and electrophoretic separation. A total of 20 samples were analyzed by using 10 microsatellite markers. It was detected a 50 alleles with an average of five alleles per locus and alleles size between 180 and 380 bp. Further were calculated statistical parameters DI, PI and PIC, which confirmed the high value of polymorphism of used SSR markers. Dendrogram was compiled, identifying the relationships between different varieties of carrots. The results were compared with scientific publications dealing with the same or very similar problems.

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