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Evaluation of qualitative and quantitative parameters of entomopathogenic fungus \kur{Beauveria bassiana} related to the inoculum production temperature
 RYBAROVÁ, Kateřina
Bachelor thesis is focusing on the entomopathogenic fungus Beauveria bassiana strain GHA, which is contained in the commercial bioproduct Botanigard. Botanigard is a product used in biological control in many countries around the world to control a wide range of pests of various plant species. In this thesis, "in vitro" (radial growth, spore production) and "in vivo" parameters (virulence) of the GHA strain of Beauveria bassiana were tested at various temperatures on different nutrient media. The most optimal temperature for radial growth of the GHA strain was 25 °C, on media with a nitrogen to carbon ratio of 20:20. The GHA strain produced the largest amount of conidia on the medium with a nitrogen/carbon ratio of 20:40 at temperature 25 °C. On the contrary, the smallest conidia production was obtained on medium PDA and nitrogen/carbon medium in ratio 5:10. The temperature of 25 °C was also the most optimal for the infection of larvae of Tenebrio molitor. Thesis also deals with the comparison of blastospores production of GHA strain with other strains of B. bassiana isolated from different geographical areas. The strain isolated in Argentina had the highest production of blastospores on MEB medium.
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