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Analysis of Calcium Pantothenat, Methylparaben and Propylparaben by Micellar Electrokinetic Chromatography Balcar, Josef ; Pospíšilová, Marie (advisor) ; Polášek, Miroslav (referee) 1. ABSTRACT 1.1. ANALYSIS OF CALCIUM PANTOTHENAT, METHYLPARABEN AND PROPYLPARABEN BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY Josef Balcar Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, Heyrovského 1203, Hradec Králové In this study, a new micellar electrokinetic chromatography (MEKC) has been developed for the simultaneous analysis of calcium pantothenat, methylparaben and propylparaben. Separations were carried out in a fused silica capillary (7,5 cm × 75 μm i.d.) at 20 kV with UV detection at 200 nm. The optimal background electrolyte was 40 mM tricine buffer (pH* 8.2) containing 50 mM sodium dodecyl sulfate as a surfactant and 20 % (V/V) methanol. Rectilinear calibration ranges were 50.0 - 500.0 mg/100ml for calcium pantothenat, 2.0 - 20.0 mg/100ml for methylparaben and 0.5 - 5.0 mg/100ml for propylparaben. The validated MEKC method was applied to the determination of the analytes in a pharmaceutical preparation. The total analysis time was <3 min. Detailed record

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