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National Repository of Grey Literature

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Identification of DNA sequence binding C/EBP and C/EBPb proteins involved in RANKL expression Dušek, Lukáš ; Křivčíková, Lucie (advisor) ; Konečná, Klára (referee) Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Lukáš Dušek Supervisor: Ing. Lucie Křivčíková, Asist. Vid Mlakar, B. Sc. microbial., Ph.D. Thesis title: Identification of DNA sequence binding C/EBP and C/EBPb proteins involved in RANKL expression Bone thinning - osteoporosis - is an increasing problem of human health worldwide. Johnell and Kanis estimated that in 2000 more than 9.0 million of osteoporotic fractures occurred - this means about 3 fractures from osteoporosis every second [1]. Because the decrease in bone mineral density (BMD) is associated with age and with the aging of the world population, the issue of bone health and especially osteoporosis is expected to emerge in its urgency. Discovery of osteoprotegerin (OPG) / receptor activator of nuclear factor kappa-B (RANK) / RANK-ligand (RANKL) pathway in late 90' gave scientists a fruitful target for future investigations about bone metabolism and revealed interesting interconnections with cardiovascular diseases, immunity and cancer survival and targeting. This study had one experimental and two theoretical goals: The experimental and primary goal was to identify transcription factors and DNA binding sites in -662 to -438 region upstream of RANKL transcription start... Detailed record

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