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A comparative analysis of the isolates of Clostridioides difficile derived from different sources.
Eretová, Veronika ; Krůtová, Marcela (advisor) ; Ježek, Petr (referee)
Clostridioides difficile infections (CDI) are generally perceived as healtcare associated infections. However, there has been recently reported an increase of CDI incidence in the community. The occurrence of C. difficile has been described also in animals, food and the environment water and soil. The aim of this thesis was to characterize C. difficile isolates derived from different sources using molecular methods. The results were discussed with available data from Czech human C. difficile isolates. A total of 135 C. difficile isolates from the following sources were analyzed: pigs n = 57, calves n = 44, horses n = 18, water n = 15 and hedgehog. Using PCR ribotyping, 22 distinct ribotyping profiles were identified, the most frequently detected ribotypes were: 033, 011, 126, 078. Both toxigenic and non-toxigenic ribotypes were detected, including binary toxin-producing strains. The most frequently detected antimicrobial resistances were to ciprofloxacin, clindamycin, and erythromycin. All ribotypes and sequence types identified in the C. difficile isolates from animals and the environment, has been found also in C. difficile isolates from humans which confirms the role of animals and the environment as a source for C. difficile. However, no epidemic ribotype 001 and 176 that dominate the current...
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