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National Repository of Grey Literature

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Study of Metastatic Processes and Response to Chemotherapy in Primary Cultures Obtained from Colorectal Carcinoma and Lymph Node Metastases Krbal, Lukáš ; Šteiner, Ivo (advisor) ; Ludvíková, Marie (referee) ; Němejcová, Kristýna (referee) Study of metastatic processes and response to chemotherapy in primary cultures obtained from colorectal carcinoma and lymph node metastases Colorectal carcinoma is one of the most common malignant diseases in the Czech Republic. Despite an implementation of primary population screening to detect premalignant lesions, the incidence and the mortality of the disease do not appear to decrease significantly. A significant percentage of patients still manifest with late (metastatic) stage of the disease. Isolation of primary cell cultures of colorectal carcinoma is a method in which a large number of tumour cells is obtained relatively rapidly under "in vitro" conditions. With a low number of passages the cells can retain most of the characteristics of the original cells "in vivo". Therefore, cell cultures are an ideal model for studying the efficacy of chemotherapeutics and chemoresistance, as well as markers involved in carcinogenesis and metastasis of colorectal carcinoma. Overall, samples from 60 patients with colon adenocarcinoma were obtained. Our intention was to take one sample from the primary tumour and another sample from the lymph node from each patient. From the primary tumours we successfully obtained 26 primocultures with a success rate of 45 % (26/58); from the collected lymph nodes we... Detailed record

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