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HPLC Analysis of Drugs
 Kouřil, Tomáš ; Kubíček, Vladimír (advisor) ; Drastík, Martin (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Tomas Kouril Consultant: Ing. Vladimir Kubicek, CSc. Title of Thesis: HPLC analysis of Drugs The diploma thesis describes selection of the most suitable conditions for determination of a two enantiomers of drugs betaxolol and bisoprolol with a method HPLC. The aim of the thesis was to find a suitable isocratic method for the substances for extraction from plasma. The chromatographic column Daicel Chiralcel ® OD-R 4,6 mm x 250 m was utilized. The best results were achieved with mobile phase consisting of acetonitrile and aqueous solution of sodium perchlorate (1 molar) in volume ratio 50:50 for betaxolol and 35:65 for bisoprolol. The column was thermostated at 25 řC. UV detection (λ = 190 nm) was applied to get a sufficient sensitivity. Tramadol and O-desmethyltramadol was tested as an internal standard. Biological samples were tested by LLE before the HPLC analyses. Furthermore, the LLE method for biological samples was tested before performing HPLC.
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