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The issue of biological exposure tests in clinical and toxicological laboratory.
WOLFOVÁ, Šárka
The aim of this work is to draw up a well-arranged paper on the use of biological exposure tests (BET) during the monitoring of intoxication by various toxic substances. In the theoretical part the advantages of BET are explained, the issue of selection of biological material, the correct time of sampling according to the duration of the exposure and the biological half-life of the toxic substances are explained. The term "maximum allowable limit" (NPL) is explained to compare BET results. The toxicity of heavy metals, organic solvents and pesticides is described. The practical part describes the BET monitoring by determining direct and indirect indicators of lead exposure using the method of atomic absorption spectrophotometry with electrothermal atomization, photometry in visible and UV area, pressure microwave mineralization and chromatographic techniques. Several cases of actual intoxication are described, as well as attempts to distort the results with the aim of achieving occupational disease due to exposure to lead. By monitoring the entire BET spectrum, this intention is revealed and objectively assessed. The relevance and timeliness of BET is highlighted, not only in the field of professional as well as non-professional exposures, and the developmental trend of gradual reduction of NPLs related to the widening of knowledge about the toxicity of monitored toxic substances.

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