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National Repository of Grey Literature

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Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization Pošustová, Veronika ; Skopalík, Josef (referee) ; Svoboda, Ondřej (advisor) This bachelor thesis is focused on an experiment based on magnetic particles SPIO for transfection of 3T3 cells. Nanoparticle surface was modified with PEI and Chitosan to optimize this method. The transfection experiment was performed twelve times, then the scanned data was processed and statistically analyzed in Matlab R2016a to determine the transfection efficiency. The theoretical part is focused on magnetic nanoparticles and their application in medicine, on transfection and in last part on a transfection of cells using magnetic nanoparticles. Detailed record

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