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HPLC method development for carotenoids determination in fruits
 Růžička, Štěpán ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Štěpán Růžička Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of the diploma thesis: HPLC method development for carotenoids determination in fruits In this thesis was optimized and developed a new HPLC method for the determination of specific carotenoids (betacarotene, lutein and zeaxanthin) content in selected species of sweet cherries. The method used Supelco Analytical RP-Amide (100 x 4.6 mm; 5 µm) column for separation and UV detection at a wavelength of 450 nm. There was used isocratic elution of the mobile phase acetonitrile:hexan:methylene chloride (96.6:1.7:1.7) at a flow rate of 1.409 ml/min during the measurement. The cherry samples were extracted to chloroform in ultrasound bath, centrifuged, and after filtration injected into HPLC system. The temperature was set at 30 řC and injected volume was 5 µl. The amount of betacarotene was quantified in a few cherry samples because of too low concentration of carotenoids.
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