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Using of modern separation methods for clinical practice Honegrová, Barbora ; Solich, Petr (advisor) ; Malíř, František (referee) ; Štěrbová, Petra (referee) Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate Mgr. Barbora Honegrová Supervisor prof. RNDr. Petr Solich, CSc. Title of Doctoral Thesis Using of modern separation methods for clinical practice This work is focused on the development of methods for the determination of vitamin A and E in biological materials using liquid chromatography. The theoretical background describes bioanalytical approaches to the determination of analytes from complex matrices. Sample preparation before analysis, chromatographic separation approaches and the validation of bioanalytical methods are discussed. The structure and biological function of target analytes are characterized. The experimental part contains three sections, two of them focus on the development of new chromatographic methods for the determination of vitamin A and E in biological samples such as serum, lipoprotein layers and breast milk. The aim is to find the most suitable conditions for the chromatographic separation of target analytes that will be used for clinical trials in University Hospital in Hradec Králové. The third section is concerned with monitoring the stability of vitamins in breast milk as well as the influence of pasteurisation and storage on levels of the target vitamins.... Detailed record

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