|
Study of chemiluminescence properties of metformin using sequential injection analysis
Horáková, Markéta ; Sklenářová, Hana (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Mgr. Markéta Horáková Consultant: PharmDr. Hana Sklenářová Ph.D. Title of Thesis: Study of chemiluminescence properties of metformin using sequential injection analysis Chemiluminescent properties of metformin were studied using a sequential injection analysis in the three systems with different composition of the reagents. The first system used potassium permanganate and sulfuric acid, the second cerium sulfate tetrahydrate and (tris (2,2 '-bipyridyl) dichloro-ruthenium hexahydrate. The third system used the N- bromsukcinimid and fluorescein. The flow rate, concentration and volumes of reagents, scheme of the measuring cycle and used solvents have been optimized into the first two systems. The concentration of N-bromosuccinimide (NBS) and fluorescein in the third system were based on data found in literature [Zhouping Wang, Anal. Lett. 2003]. The volume and speed were detected automatically using the program FaFSIAOptim, created the Department of Analytical Chemistry. Scheme of the measuring cycle was optimized in the same way as in the previous two systems. During the optimization, and subsequent measurements revealed that the only suitable system is the third system with NBS and...
|
guest ::
