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Analysis of natural compounds by capillary isotachophoresis and capillary zone electrophoresis.
Černá, Veronika ; Polášek, Miroslav (referee) ; Pospíšilová, Marie (advisor)
It has been designed tITP - CZE method with a reversed electro-osmotic flow for the analysis of seven organic acids of natural origin. It was monitorated the effect of boric buffer concentration, pH effect, the addition of organic solvent, the addition of cyclodextrin, concentration of NaCl as a stacker, and injection time. Measurements were made in voltage - 30 kV in capillary with inner diameter 50 μm and effective length 68 cm. Optimum separation conditions were found at a concentration of 125mM boric buffer, pH 8,2, the addition of 0,001% hexadimethrinbromid and addition of 10mM and 2,6-di-O- methylcyklodextrin. The stacker was used at a concentration of 200mM NaCl and injection time was 90 s. Intracapillary thermal gradient from 20o C to 24řC during electrophoresis has yielded improved resolution for the analytes.
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