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Bacteriological examination of blood cultures
 MAJEROVÁ, Natálie
The aim of this bachelor thesis is the introduction of possibilities of hemoculture examination, assuming the methodology of evolving the manual and automated hemocultures in tha Stafila laboratories and at the Department of Bacteriology in the Hospital of České Budějovice. Then it deals with the comparison of those two systems and learning the basic interpretation of results. In the theoretical part I focus on blood circulation infections, sepsis and its diagnostics and nozocomial infections.The last part delas with the most frequent bacterial causers of blood circulation infections. The practical part focuses on the approach of hemoculture process, firstly on the preanalytic part which includes the sample collection and its transport. Secondly there is the analytic part where cultivation bottles are described as well as the basic methods for bacteria certification such as making the microscopic sample and bacteria cultivation. The next part delas with the determination of a bacteria kind and its antibiotics sensitivity.In the last part there are results of cultivation of the followed up file from the both methods. Positive findig occuresd in 15% of taken hemocultures.
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