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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Preparation of fluorescently labeled substrates and determination of transglycosylation activity in plants
 Velínská, Kamila ; Ing.Kristína Kováčová (referee) ; Firáková, Zuzana (advisor)
The bachelor's thesis was focused on preparation of fluorescently labeled oligosaccharides and detection of transglycosylating/heterotransglycosylating activities of enzymes from dicotyledons. As the model organisms nasturtium and parsley were used. The quality and composition of labeled acceptor substrates was monitored by TLC and MALDI-TOF MS. The presence of enzymes in the plant seeds was analysed by SDS electrophoresis and IEF-PAGE. The paper method and HPLC were used for the detection of transglycosylating activities. The obtained results suggest that enzymes catalyzing heterotransglycosylating reactions are in the seeds of studied plants.
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