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Monitoring of wine yeasts population during fermentation of wine cider
Krätschmerová, Kateřina ; Vojtíšková, Marie (referee) ; Vránová, Dana (advisor)
This thesis deals with identification of wine yeasts isolated during fermentation process of wine cider and grapes of Sauvignon grape variety, grown in the integrated vineyard. The identification and taxonomic classification is faster and easier due to the progress of molecular methods. In this thesis PCR-RFLP method was used for identification of yeasts. Sequences of DNA specific for each species were analysed. These sequences were amplified by means of PCR method and by using ITS1 and ITS4 primers. In following step, they were put through the restrictiction analysis with five restriction endonucleases. Fragments of DNA were separated by horizontal electrophoresis. The electrophoreograms were evaluated by BioNumerics software and final dendrogram representing genetics similarity of isolated yeasts was created by using UPGMA claster analysis. The basic information about yeasts and their identification by molecular methods are described in the theoretical part of this thesis.
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Influence of grape growing methods on yeasts community
Jiříková, Ivana ; Vojtíšková, Marie (referee) ; Vránová, Dana (advisor)
This diploma thesis has analyzed the effect of organic wine-growing on the wine yeasts population. The wine yeasts were isolated from the Pinot Noir variety. They were identified by the molecular biological method PCR-RFLP. The theoretical research compiles basic information on yeasts, knowledge about the red wine production as well as information on molecular biological methods. The experimental part utilizes the 5,8S-ITS rDNA specific segment for analysis. The segment was amplified using the ITS1 and ITS4 primers and subjected to restriction analysis. The restriction analysis has used these restriction endonucleases - HaeIII, HinfI, Taq?I, AluI and MseI. The BioNumerics software was then used to compare genetic similarity between the isolated yeasts and these were taxonomically classified.
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Senzorické hodnocení vybraných druhů ovocných vín
PRÁŠEK, Jiří
The aim of this thesis was to prepare at least three samples of fruit wines and make a sensory evaluation of them within a group of evaluators. The results of the sensory evaluation of fruit wines bought in the market were added. The sensory analysis of the fruit wine samples was done at the Faculty of Agriculture at the University of South Bohemia in České Budějovice. Evaluators (n= 61; 21 to 55 years old) made a perception (ranking and sensory profile) of the fruit wine samples. The best self-made fruit wine was rosehip wine (fruit of Rosa canina). The worst self-made fruit wine was blackthorn wine (fruit of Prunus spinosa L.). The assessors did not register any big differences during the analysis of the commercially made fruit wines, therefore the variability in evaluation depended on the individual preferences. The complete impression of evaluated fruit wines mainly consisted of the results from analysis of the aroma and the taste of those samples.
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Influence of grape growing methods on yeasts community
Jiříková, Ivana ; Vojtíšková, Marie (referee) ; Vránová, Dana (advisor)
This diploma thesis has analyzed the effect of organic wine-growing on the wine yeasts population. The wine yeasts were isolated from the Pinot Noir variety. They were identified by the molecular biological method PCR-RFLP. The theoretical research compiles basic information on yeasts, knowledge about the red wine production as well as information on molecular biological methods. The experimental part utilizes the 5,8S-ITS rDNA specific segment for analysis. The segment was amplified using the ITS1 and ITS4 primers and subjected to restriction analysis. The restriction analysis has used these restriction endonucleases - HaeIII, HinfI, Taq?I, AluI and MseI. The BioNumerics software was then used to compare genetic similarity between the isolated yeasts and these were taxonomically classified.
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