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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Optimalisation of DNA isolation from opium poppy plants and poppy products.
 ZAHRADNÍK, Václav
The thesis deals with the evaluation of selected DNA isolation methods on the selected samples of poppy, to evaluation the quality and the quantity of isolated DNA and select methods, which could be used in the future for testing forgery poppy in the food. There are mentioned methods of isolation and methods of evaluation in the literature research. The methodology is focused on isolation using kits and conventional methods (CTAB-PVP), evaluation of obtained samples by spectrophotometric measurement and PCR reaction followed by gel electrophoresis. It is obvious from the results that classical CTAB-PVP methods modified for plants appear to be the best methods. However, I do not recommend these methods because of the difficulty at work and I am inclining towards the company-supplied kits I have tested.
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