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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Metody dekontaminace rekombinantních proteinů od bakteriálního lipopolysacharidu
 CHARVÁTOVÁ, Lucie
In this work, three decontamination methods based on two-phase micellar system and affinity chromatography were used to decontaminate recombinant proteins from bacterial lipopolysaccharide and to determine which method is the most effective. The efficiency of this method was measured using various recombinant proteins at several protein concentrations. Three different assays, two chromogenic and one fluorogenic, were used to measure the concentration of endotoxins in samples. The most accurate method for measuring the concentration of endotoxins was determined.
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Vliv střevní mikroflóry na endotoxinový šok
 Hrnčíř, Tomáš ; Kobayashi, K. ; Flawell, R. A. ; Hudcovic, Tomáš ; Kozáková, Hana ; Štěpánková, Renata ; Tlaskalová, Helena
Endotoxin shock is the most severe complication of sepsis. Lipopolysaccharide (LPS) or endotoxin, a structural component of gram negative bacteria, is a major effector and can activate the innate immune system and lead to multiple organ failure, termed endotoxin shock. In our project we study the effect of intestinal microflora on the susceptibility to endotoxin using a model of germ-free and specific pathogen free mice
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