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Differentiation of different types of eggs by mass spectrometry
Švárová, Markéta ; Kučková, Štěpánka (advisor) ; Rusek, Martin (referee)
The aim of the diploma thesis was to distinguish bird species based on the expected different protein composition of eggs and their individual components using mass spectrometry. The obtained results could be possibly used to identify the animal origin of egg materials used in works of art. For the research, components of the eggs (yolk, egg white and their mixture) of nine available bird species were collected and dried - pheasant (Phasianus colchicus), domestic goose (Anser anser domesticus), domestic duck (Anas platyrhynchos domesticus), muscovy duck (Cairina moschata), chicken (Gallus gallus f. domestica) - four different specimens, Japanese quail (Coturnix japonica), pampas rhea (Rhea americana), red ore (Alectoris rufa), speckled guinea fowl (Numida meleagris). The samples were digested by the enzyme trypsin and prepared using ZIP-TIP for the next measurements by MALDI-TOF (Matrix-Assisted Laser Desorption / Ionization - Time of Flight) mass spectrometry. The obtained data were evaluated by Principal Component Analysis (PCA). The used method showed that the most bird species can be distinguished by yolks (with an approximately 95% success rate) and more than half of the species by egg white proteins (success rate 83%) and by the egg white and yolk mixtures (success rate 80%). Two samples of...
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