|
|
Individual growth and variability of early development stage of creeping water bug Ilyocoris cimicoides (Heteroptera: Nepomorpha:Naucoridae)
PILAŘOVÁ, Simona
This thesis investigates an influence of environmental variables (temperature and food availability) on development and growth of the first three nymphal stages of Ilyocoris cimicoides Linnaeus, 1758 Individuals were housed individually in experimental cages from eklosis into adulthood. Experiments were carried out in a combination of four different temperatures (17 ?, 19 ?, 22 ? and 25 ? C) and two different modes of food availability (full-fed every day, red-fed every other day) (in eight treatments). The temperature significantly influenced the length of nymphal development. According to our expectation, shortest development was achieved in individuals reared at 25° C. Development period was prolonged by decreasing temperatures. Unpredictably, the nymphs of the 3rd instar had longest development at the lowest temperatures (17 ? C) whereas nymphal development of the 1st and 2nd instars ran faster Both, the lowest- (17 ? C) as well as the highest used temperature (25 ? C) are probably very close to the temperature limits, that corresponds to the limits of postembryonic development. The optimum temperature for development was 22 ? C. Furthermore, it has been found that food availability should also significantly affect growth and development. Surprisingly, less mortality was observed in individuals reared in reduced feeding treatment, except the only case - nymphs reared in low temperature (19 ? C), where higher mortality was found in individuals reared at the same food availability.
|
|
|
Cold hardiness of larvae of the fruit fly, \kur{Drosophila melanogaster} (Diptera: Drosophilidae)
KORBELOVÁ, Jaroslava
We assessed survival of larvae of the fruit fly, Drosophila melanogaster at low temperatures (0°C and 5°C) after rearing them under seven different acclimation regimes. Larvae that have developed in a standard diet at 25°C showed 50% mortality after 12,6 min of the exposure to 0°C (Lt50 = 0.21 h). In contrast, larvae that have developed in a diet enriched with glycerol at 15°C, and were cold acclimated at 5°C during last two days of their development, had Lt50 = 38.6 h. It means that it was possible to increase the Lt50 at 0°C more than 180-fold using simple manipulations with rearing temperatures and diet composition. The physiological differences in duration of larval development, fresh mass, dry mass, hydration and total contents of proteins, lipids and glycogen between the larvae belonging to different acclimation variants are described. The samples for future detailed metabolomic analysis were prepared.
|
guest ::
