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Metabolomics of cerebrospinal fluid in multiple sclerosis
Židó, Michal ; Štětkářová, Ivana (advisor) ; Uher, Tomáš (referee) ; Pavelek, Zbyšek (referee)
Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease that damages white matter of the central nervous system (CNS). The exact cause of MS is still unknown, but various environmental and genetic factors are believed to be involved in its development. The pathophysiology of MS is a very complex. MS is currently an incurable disease, but specific therapy can be used to influence its course. Metabolomics is a systematic study that uses an analytical chemistry approach to profile endogenously small molecule metabolites present in the examined preparation. The main goal of this dissertation was to determine significant differences in the metabolome of the cerebrospinal fluid of patients in the initial stage of MS compared to controls. Furthermore, we performed a correlation analysis of these results over time with the clinical condition of the patients in the form of EDSS values. Subsequently, as part of further analyses, we also evaluated other potential biomarkers of MS, more precisely evoked potentials (EP), neurofilament light chains (NfL) and glial fibrillary acidic protein (GFAP). In total, we collected CSF samples from 40 patients in the early stages of MS, and CSF samples from 33 healthy controls. Subsequent analysis of these samples was performed using high-performance liquid...
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Utilisaation of Physiotherapeutical Procedures and Methods in Patients with the Multiple Sclerosis
Barešová, Michaela ; Puršová, Martina (advisor) ; Palacký, Vít (referee)
The title of thesis: Utilisaation of physiotherapeutical procedures and methods in patients with the multiple sclerosis. Abstract of thesis: This work is divided into theoretical and practical part. The content of the firt part is a study of theoretical works related to multiple sclerosis. The main chapters are devoted to history of multiple sclerosis, the definition of why and how the disease arises, the types of disease, clinical presentation, diagnosis, therapy, desce incidence and complications that can occur to people with this disease. Finally, I looked at various physiotherapeutic procedurs and methods related to this disease. In the practical part of the thesis , a qualitatice research metod - cause study - was used. The research was conduted at the Center for demyelination disorders (MS center) at the Charles Square. The test set consisted of two probands with a diagnosis od cerebro-spinal multiple sclerosis, specifically of the relapsing - remitting form of disease. These diagnoses were determined by the attending physician of the probands. During the physiotherapy I used elements of the Bobath koncept. The practical part also inculdes twi case reports and subjective evaluation of physical therapy that were recorded by the patients after physiotherapy. The results are processed in the...
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