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Identifikace forenzně významných druhů hmyzu s výskytem v chladných měsících
 Skoupá, Kristýna
The identification of insect inhabiting dead bodies is particularly relevant in the area of forensic science, especially for post-mortem interval determination, as evidence of post-mortem manipulation of the body, evidence of the presence of toxic substances, etc. This work deals with the determination of forensically important insects occurring in cold months and extreme conditions based on molecular-genetic analyzes. For this purpose, were established 3 experimental fields in Brno-venkov and Blansko district and at the bottom of the Macocha Abyss with a specific cave microclimate. DNA isolation was performed, amplified the cytochromoxidase I gene and sequenced this part of mitochondrial DNA of insect specimens. Then the sequence was compared to the BLAST and BOLD databases, the BOLD showing a higher percentage of matching. This way, were identified 24 insect species from a total of 13 different families. The most of the trapped insects formed Calliphora vicina and Calliphora vomitoria, typical representatives of winter necrophagous fauna.
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