|
|
Involvement of vibrational spectroscopy in the in-situ analysis of PHA in bacterial biomass.
Kevélyová, Barbora ; Samek, Ota (referee) ; Sedláček, Petr (advisor)
The aim of this thesis was the study of polyhydroxyalkanoates in bacterial biomass using the ATR-FTIR method. Furthermore, the PCA method was chosen to evaluate the measured data and observe correlations or differences among the spectra of various biomass samples. The bacterial producents were Aneurinibacillus thermoaerophilus H1, AH30 and AFn2, Cupriavidus malaysiensis DSM 25816, DSM 19416 and DSM 19379 and three strains of Schlegelella thermodepolymerans M 15344, DSM 15264 and LMG 21645. Various heteropolymers with different monomer content and total PHA content were investigated. The monomers in the samples were 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxyvalerate, 5-hydroxyvalerate and 4-hydroxyhexanoate. As the reference method for accurate quantitative and qualitative determination of polyhydroxyalkanoates in the samples, gas chromatography was performed. The spectra obtained by the FTIR method were compared and significant peaks related to the qualitative properties of PHA, namely monomer composition and crystallinity, were searched for. The PCA statistical method was successfully applied to the collected data from FTIR, with the help of which the qualitative differences between the samples were monitored. The most significant differences were observed in the presence of monomers 4-hydroxybutyrate and 4-hydroxyhexanoate, and they were related to the crystallinity of the samples. The analysis did not show potential for semi-quantitative determination of PHA in biomass. Differences between microorganisms were not observed. The procedure for evaluating FTIR spectra using PCA could in the future be used in the study of biomass containing various monomeric units, especially hydroxyvalerates in case of their higher content within the sample.
|
|
|
Spectral Analysis of EEG Signal
Miklenda, Petr ; Orság, Filip (referee) ; Kupková, Karolína (advisor)
This bachelor thesis is focused at the EEG signal analysis using the Fast Fourier Transform. The theoretical part consist of EEG signal description, its creation and selected method for signal analysis. The practical part presents program for recording data from EEG headset and displaying the Fourier spectrum.
|
|
|
Spectral analysis of the EEG signal
Blatný, Michal ; Kozumplík, Jiří (referee) ; Hrozek, Jan (advisor)
Master’s thesis deal about electroencefalography, measurement EEG signals and analysis measuermed signals. Project contains two basis practical parts. Firts part contain two PC’s programs that’s are used to fundamental analysis to frequence-domain and visual display of brain mapping created with Matlab. Second chapter of practical parts includes two PC’s programs created with LabView. First of them is the EEG biofeedback making use for advanced analyses and second program is used to detection segment of stacionarity.
|
|
|
Dynamic analysis of structure loaded seismic loads
Janošková, Lenka ; Nevařil, Aleš (referee) ; Vlk, Zbyněk (advisor)
In this diploma thesis the seismic load on two different models is solved – the first model is a beam hall and the second model is a multistory building. The calculation of seismic load was performed according to standard ČSN EN 1998-1 (Eurocode 8) using the response spectrum analysis and the method of equivalent static forces (substi-tute load). For the combination of seismic responses in different directions are used SRSS and CQC rules. Models and calculations were solved in a student’s version of program RFEM 4.10 (Ing. Software Dlubal, s.r.o.). The comparison of mentioned methods in each models, also the comparison of combinative rules in calculation and the final evaluation of seismic responses on both of models are the subject of this thesis.
|
|
|
Spectral analysis of flute organ whistles and their comparison
Sommer, Tomáš ; Michálková Slimáčková, Jana (referee) ; Jirásek, Ondřej (advisor)
This work is focused on the problems of spectral analysis of flutes stops in organ pipes. The first part of this work is about terminology of organ pipes. This theoretical part is also focused on the problem of harmonic analysis with the use of mathematical signal processing methods, which allows this analysis. The second part of this work deals with measuring methods of specific organs and acquiring sound samples and their evaluation and spectrum description.
|
|
|
Analysis of Time-Varying Spectrum of Pianos
Smělý, Pavel ; Rajmic, Pavel (referee) ; Jirásek, Ondřej (advisor)
This work focuses on an analysis of a time-varying spectrum of pianos. The beginning of the work deals with the gradual evolution of the piano, aiming at timbre and design, and it analyzes in detail its individual parts and their influence on the sound spectrum. The theoretical part also concentrates on the issue of harmonic analysis in both the musical conception of timber and the usage of mathematical signal processing methods that make this analysis possible. Another part deals with practical tasks of the work such as a description of an application and functions created in the interface of MATLAB for exploring the time-varying spectrum of tones, description of the method and procedure of the measuring of three concert grand pianos. The work also analyzes, by supporting it with practical examples and by comparing the measured pianos, the most important acoustic phenomena that originate from the production of tones in the instrument; it describes the timber of individual instruments, and it gives recommendations.
|
|
|
The spectral and directional analysis of xylophone, vibraphone and glockenspiel
Hoffman, Pavel ; Indrák, Michal (referee) ; Jirásek, Ondřej (advisor)
Bacherlor‘s thesis deals with spectral analysis and directional characteristics of idiophonic instruments, xylophone, vibraphone and glockenspiel. It analyzes various details during spectral analysis, that could have an effect on the spectrum of a given instrument, and especially on its inharmonicity, thus analyzing its effect on the hardness of mallet, dynamics and the location of the mallet striking the bars (stones, wooden plates). It examines the changes in the harmonic range and the formant areas of instruments. The thesis further describes the mallets, the instruments themselves, theoretical base and the means by which the sound samples were acquired and processed.
|
|
|
The tri-band fluorescent reader
Škrabalová, Denisa ; Čolláková, Jana (referee) ; Dostál, Zbyněk (advisor)
This bachelor thesis deals with the issue of laboratory testing and design of an optical instrument called a tri-band fluorescent reader, which is designed for fluorophores Alexa Fluor 488, Alexa Fluor 555 and Alexa Fluor 647. It contains a theoretical part with key knowledge not only in the natural sciences, such as physics, chemistry, biology, but also the current technical capabilities that are necessary for the practical part. In the practical part is drafting the objectives of the thesis, which are the measurement and evaluation of the samples examined using a monochromator, acquisition and evaluation of images using the existing system, build spectral analysis throughput, create a simulation of custom solutions and create a system engineering design. The mechanical model of the reader assembly was made after measuring the samples and the device and creating the simulation.
|
|
|
Analysis and comparison of spectra in different piano models
Peloušek, Tomáš ; Přikryl, Lubor (referee) ; Jirásek, Ondřej (advisor)
This work focuses on the topic of analysis of the time varying spektrum of the piano. The introduction deals with the historical development of the piano, of its individual parts and the way they influence the tone colour. The subsequent theoretical part is focused mainly on the topic of spectral analysis in the musical concept of sound colours. In the following part a measuring of particular musical instruments is described, these experiments being conducted in an anechoic chamber provided by the instrument producer. It also lists the various methods and calculations used in the process and the conclusion presents analysis of acquired samples.
|
|
|
Digital Signal Processor based Baby Cry Detector
Sobotková, Šárka ; Rozman, Jiří (referee) ; Sekora, Jiří (advisor)
The thesis discusses the baby cry. Processing of literary research in dealing with children crying and processing of audio signals. Based on information gathered propose an algorithm for detecting child's cry, which was verified using Matlab programming environment. Based on the identified parameters of the input signals and the proposed algorithms was selected appropriate digital signal processor. The hardware device was realized to detect and initialize the child crying, and its functionality has been verified in the recorded sound recordings child crying.
|
guest ::
