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Optimization of litter size in rodents
Cingelová, Alexandra ; Dušek, Adam (advisor) ; Sedláček, František (referee)
Optimization of litter size can be defined as adjustment of litter size according to actual environmental conditions or condition of mother to reach a compromise between the maximum number of offspring and their individual fitness. Rodents are an ideal taxon to study this topic because of their enormous interspecific variability in life strategies across the whole order. The variation in litter size among rodent species was influenced by countless ecological and social factors during their evolution. These include the geographical location of the breeding populations, various life and reproductive strategies such as r-/K-strategy, the presence of altricial or precocious offspring, various ways of life, the presence of water in the environment, predation rate, hibernation, and sociality. Litter size can be adjusted already before fertilization, for example by choosing a partner, by timing reproduction, condition, age and social status of the mother. Genetic factors affecting litter size are, for example, MHC glycoprotein polymorphism and t-haplotype. Reduction of the litter size may occur before insemination by influencing the ovulation rate, female reproductive hormone levels and exposure to the stressors, during implantation by selective abortion, and postpartum by infanticide, siblicide, and...
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Effect of cryopreservation on mouse sperm.
Veselá, Kateřina ; Hortová, Kateřina (advisor) ; Pěknicová, Jana (referee)
Cryopreservation or freezing of sperm in the reproductive biology is still actual topic. Today is the only method used for sperm storage, whether for the purposes of assisted reproduction, or for scientific purposes. However, this method has a negative impact on such stored cells and is therefore still a subject of many studies. Among the main causes of sperm damage in rodents there are inappropriately selected cryoprotective agents, poor or no elimination of oxidative stress generated during cryopreservation, as well as a poorly chosen speed and temperature of freezing. Correctly chosen procedures and the composition of the media in which sperm are stored, can significantly affect the quality of sperm. This bachelor theses focuses on the influence of cryopreservation on mouse spermatozoa. The main impact of this method includes the effect of freezing on sperm DNA, plasma membrane, acrosome, and sperm motility.
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Optimization of litter size in rodents
Cingelová, Alexandra ; Dušek, Adam (advisor) ; Sedláček, František (referee)
Optimization of litter size can be defined as adjustment of litter size according to actual environmental conditions or condition of mother to reach a compromise between the maximum number of offspring and their individual fitness. Rodents are an ideal taxon to study this topic because of their enormous interspecific variability in life strategies across the whole order. The variation in litter size among rodent species was influenced by countless ecological and social factors during their evolution. These include the geographical location of the breeding populations, various life and reproductive strategies such as r-/K-strategy, the presence of altricial or precocious offspring, various ways of life, the presence of water in the environment, predation rate, hibernation, and sociality. Litter size can be adjusted already before fertilization, for example by choosing a partner, by timing reproduction, condition, age and social status of the mother. Genetic factors affecting litter size are, for example, MHC glycoprotein polymorphism and t-haplotype. Reduction of the litter size may occur before insemination by influencing the ovulation rate, female reproductive hormone levels and exposure to the stressors, during implantation by selective abortion, and postpartum by infanticide, siblicide, and...
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Effect of cryopreservation on mouse sperm.
Veselá, Kateřina ; Pěknicová, Jana (referee) ; Hortová, Kateřina (advisor)
Cryopreservation or freezing of sperm in the reproductive biology is still actual topic. Today is the only method used for sperm storage, whether for the purposes of assisted reproduction, or for scientific purposes. However, this method has a negative impact on such stored cells and is therefore still a subject of many studies. Among the main causes of sperm damage in rodents there are inappropriately selected cryoprotective agents, poor or no elimination of oxidative stress generated during cryopreservation, as well as a poorly chosen speed and temperature of freezing. Correctly chosen procedures and the composition of the media in which sperm are stored, can significantly affect the quality of sperm. This bachelor theses focuses on the influence of cryopreservation on mouse spermatozoa. The main impact of this method includes the effect of freezing on sperm DNA, plasma membrane, acrosome, and sperm motility.
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