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National Repository of Grey Literature

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Metody identifikace rostlinných virů - historie a současnost Kretková, Kateřina This work deals with a general summary of methods for identification of plant viruses. The first part introduces the issue of plant viruses, sumarizes the historical discoveries and explains taxonomy. The second part focuses on the methods of virus detection. The work mentions the characteristic symptoms of viral infection, which may not lead directly to the identification of the pathogen. For the precise identification of the virus we use methods physical, serological and molecular. In the future, we can assume the development of molecular technologies along with a decrease in claims to expertise by constructing a detector, which evaluates the data obtained independently of the human factor. Detailed record

Methodology of resistance evaluation of plum, Prunus domesica L., clone C5 to the Plum pox virus and mixed infections with another two viruses Polák, Jaroslav ; Kumar, Jiban ; Jarošová, Jana The methodology encloses the procedure of preparation and ínoculation of transgenic plants of plum with the individual viruses, the maintenance of field orchard, the evaluation of virus symptoms in leaves, serological and electronmicroscopical detection of viruses in leaves of plum trees, determination of relative concentration of PPV by DAS-ELISA, detection of PPV-Rec, ACLSV and PDV by RT-PCR, and determination of risk of recombinations between PPV-Rec and the homologous transgenic transcript in P. domestica, clone C5. Fulltext: PDF Detailed record

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