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Výskyt a biologické vlastnosti Cryptosporidium avian genotyp III
ZIKMUNDOVÁ, Veronika
The diversity of Cryptosporidium spp. in captive parrots were studied. Faecal samples of pet birds from Psittaciformes were screened for the presence of Cryptosporidium by microscopy and sequencing of the small-subunit rRNA, actin and 70 kDa heat shock protein genes. Cryptosporidium infections were detected in 27 of 402 Psittaciformes. Cryptosporidium avian genotype III as the most prevalent Cryptosporidium (22), while C. baileyi (4) and C. avium (1) were detected in four, respectively two cases. Cryptosporidium avian genotype III was infectious for cockatiels under experimental conditions, with prepatent period of six days post-infection (DPI), but not for budgerigars, chickens or SCID mice. Experimentally infected cockatiels remained infection for the duration of the study (30 DPI), with an infection intensity ranging from 4,000 to 30,000 oocysts per gram (OPG). Naturally infected cockatiels shed oocysts with an infection intensity ranging from 10,000 to 30,000 OPG. Cryptosporidium avian genotype III infects the proventriculus and ventriculus, and oocysts measure 7.4×5.8 m. None of the birds, infected with Cryptosporidium avian genotype III, developed clinical signs. Genetic and biological data support the establishment of avian genotype III as separate species of the genus Cryptosporidium and we propose the name Cryptosporidium proventriculi sp. n.
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