|
|
Molekulární markery pro detekci genetické variability přírodních populací forenzně významných druhů bzučivkovitých (Calliphoridae, Diptera)
KLOJDOVÁ, Martina
This thesis was focused on suitability of selected molecular markers for detection of genetic variability of four species from the family Calliphoridae important in the forensic entomology. A set of eight markers, both mitochondrial (COI, ND6, CytB, CR) and nuclear (RP S12, RP S13, RP L12, PB2), were applied on samples from natural populations of representative species (Calliphora vicina, Lucilia caesar, Lucilia sericata, Phormia regina) most common in the Czech Republic. Level of detected variability was evaluated and compared, both with respect to the particular species as well as their geographic origin.
|
|
|
The effect of previous freezing of cadavers on the following colonization by flies of the family Calliphoridae
Černá, Monika ; Barták, Miroslav (advisor) ; Olekšáková, Tereza (referee)
The aim of the thesis was to rally available information and draw up a review of literature data on the topic of how freezing of a carcase or its storage at low temperatures affects its following colonization by insects while open exposition.
Forensic entomology is one of the special and ever-developing field of criminology. It uses a knowledge of the biology, ecology and especially the life cycles of insect, mainly flies and beetles, to assist in the investigation of violent crimes in estimating of post mortem interval (PMI) or determining the cause of death. Insect colonization openly exposed corpses go through 5 to 8 successional waves depending on the surrounding environment. One of the important factors is the temperature which affects the decomposition process, where decomposition processes are longer at lower temperatures and on the contrary at shorter at higher temperatures. Temperature has a similar effect on development of insect, which develops only within the effective temperatures, whose values vary by species.
The study performs an experiment which should verify whether insect is able to oviposit on the frozen carcasses and whether pre-frozen carcasses slow the subsequent colonization by flies of the family Calliphoridae. During the experiments, a total of twelve pieces of domestic fowl (Gallus gallus f. domestica L.), weighing about 1.5 kg, were sacrificed in the same way, and a half of them was frozen before the exposition. Experiment results showed that the percentage of each species in both variants differed. Frozen and fresh cadaveres differed in the total nu mber of individuals (larvae), which developed on them, and the way in which the dead bodies decayed.
|
|
|
The influence of height above ground on colonization of carcasses by blow flies (Calliphoridae)
Mudrochová, Michaela ; Barták, Miroslav (advisor) ; Olekšáková, Tereza (referee)
The forensic entomology is a special discipline of criminology which uses knowledge about insects cycles, mainly Diptera and Coeloptera, to determine the post-mortem interval. The post-mortem interval is time from death to finding of a corpse.
In the Czech Republic, there wasn´t implemented any terraneous experiment that would prove the truth or disprove about the influence of height above ground on colonization of carcasses by blow flies (Calliphoridae). Experimental part was directly focused on that. For the experiment, there was used a building with six floors, precisely it was used balconies on the third and sixth floors and bush at the ground level in the shadow in front of the building. Meat baits which represented cadavers were on the all floors. Baits were used for colonization and breeding species of flies which were determined afterwards. Finally, obtained data were evaluated for their utilization in forensic entomology.
|
guest ::
