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Assessment of small machinery for processing and soil cultivation acording to selected indicators of exploitation
PAVELA, Josef
The first part of thesis is written as a literary review, which deals with the basic information of soil composition, the importance of soil in agriculture and the methods of soil tillage. It follows dividing of small tractors, machinery and tools for primary soil tillage, the soil preparation before sowing and planting and the soil cultivation. A literary review is concluded by an analysis of exploitation, economic and environmental indicators. The second part of thesis is formulated as the methodical way The methodology deals with measurement of operational tests, which means the time-frames measuring of selected rotary tillers. The thesis presents the results of measuremens of selected rotary tillers MOUNTFIELD MS 16 IN and VARI IV GLOBAL and compares the them. The conclusion of the thesis deals with an evaluation of the results, achieved by measurement.
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Investigation of chlamydia urogenital tract
BENDOVÁ, Radka
The subject of this bachelor paper is chlamydiae examination in urogenital tract via the method of cultivation. The aim of this paper is to test the cultivation method of diagnosis Chlamydia trachomatis on a tissue of culture, to evaluate the outcome according to individual criteria in a given period and to compare the outcome with the literary data. The virology laboratory in hospital in České Budějovice, a.s. Is equiped for the culture tissue preparation and for this method they use uncommon line of cells BGM, which is not commonly used in the world. In this department this line has proven and so they use it since 1985, when they received it. This paper informs about the present situation of chlamydiae, their clasification according to phylogenetic and genome bacterial analysis, about their occurence, growth cycle and multiplication. The paper also focuses on chlamydiae infections divided into ocular and urogenital infection and their treatment. The overview of present issues is finished by the methods of evidence, direct and indirect evidence. In the direct evidence there are described the issue cultures their preparation and cultivation. This method was verified in the practical part of my bachelor paper. For this bachelor paper I was provided with samples from urogenital tract which were evaluated in three categories: positive, negative and imposible to evaluate (samples were overgrown with bacteria or yeast cells and it was imposible to evaluate them). They were mostly smears from urethra and cervix (at women) or from urethra only (at men). The number of all samples was 159 and they were examined during the period from 18th October to 5th December 2011. Chlamydiae grew on issue cultures for 48 hours and then they were died with modified colouring Macchiavella. 127 examples (79.9%) coloured by this technique were evaluated from the total number. Ambiguous samples were coloured by the monoclonal antidote against Chlamydia trachomatis. 32 samples (20.1%) were coloured using this technique from the total number.I also evaluated the samples according to age. Unusually high was positivity (42.2%) at women at the age of 26-46. Which includes the highest number of samples (54.2%) of women from the total number. It mostly concerned pacients with gynaecological difficulties who were never examined and treated or they had a chronical infection which can be treated with difficulties and inclinates to relapse. At men the highest number of samples (56.1%) from the total number was at the age of 26-46 but only 13% were positive. The difference is given by the size of samples and also at men the occurence of chronical infection is less common.
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Blastocystis in domestic birds
HRDLIČKOVÁ, Jaroslava
During 2010, faeces samples for parasitology research oriented on Blastocystis were collected from an anonymous farm. A total of 55 samples were collected (of them for 10 faeces samples from hens, ducks and geese and 25 from pigeons). The samples were cultured in Dobell-Leidlaw medium and viewed by light microscopy. However, this method led to only two foundings of Blastocystis (one from hen, the other from pigeon). Thus, PCR and nested-PCR with specific primers were later used for better detection of Blastocystis. The samples for PCR detection were not collected from the aforementioned farm, but they originated from a collection of isolated DNA samples that was available on the Parasitology institute of AS CR. The results of PCR were checked after electrophoresis and verified by sequenation. The obtained sequences of bird-isolated Blastocystis were phylogenetically analysed and as described to subtype 7.
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