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	Study of penetration of selected substances from elderberry extracts through biological membrane Škubalová, Zuzana ; Němcová, Andrea (referee) ; Vespalcová, Milena (advisor) This Diploma thesis focuses on the study of penetration of selected phenolic substances contained in extract from black elder (Sambucus nigra) via modelling systems represented by two types of synthetic membranes and a real system represented by a biological membrane. As a biological membrane, we have chosen skin from pig´s earlobe. Experiments of transdermal absorption and absorption through synthetic membranes were conducted through vertical (Franz´s) cells. Selected substances contained in the extract from black elder were rutin and chlorogenic acid. The main focus of this work was to determine how readily, or if at all, these two selected phenolic substances penetrate the skin and selected synthetic membranes. The percentage of absorbed amount of the substance was determined by HPLC analysis. Detailed record
	Experimental study and modelling of the transdermal penetration of active species from gels Palanová, Veronika ; Opatřilová, Radka (referee) ; Sedláček, Petr (advisor) This diploma thesis deals with design and experimental study of transdermal transport of pharmaceutically active agents from gel matrices, which contain humic substance in its structure. A model absorption membrane was represented by the skin of pig´s earlobes. The study of the release of active substances and Lignohumte was performed due to the vertical diffusion cells. The amount of released humic substance was characterized by UV-VIS method and the amount of released active agent from gel matrix was determined by HPLC-DAD. The most interesting finding of this diploma thesis was that Lignohumate enhances transdermal transport of active agents and supports their release from gel samples to the particular environment. Detailed record
	Laboratory Measurements of Skin Conductance Slouka, Petr ; Harabiš, Vratislav (referee) ; Sekora, Jiří (advisor) This thesis contains an introduction to anatomy and physiology of the skin highlighting its barrier function. The barrier function of the skin enables it to separate internal organism from external environment. thermore, the electrical properties of the skin are described and possibilities of skin conductance measurement are discussed. The thesis presents a design of a device for the skin conductance measurement as the method of evaluation for barrier function. Alternating current is used for measurement during iontophoresis for evaluation of skin conductance. Circuit design and circuit board are included with a list of parts. The designed device was realized and in the end it was tested and the results were discussed. Detailed record

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