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	Effect of selected exogenous and endogenous factors on transformation efficiency SKALICKÝ, Tomáš The main goal of this bachalor thesis was to explore the possibility to use an ultrasound sonication as a way to increase the transformation efficiency of flax. Another part of this thesis is devoted to the searching for the seed sterilization method and finding an optimal concentration of kanamycin antibiotic useful for the selection of transformed plants. Detailed record
	Possibilities of the green fluorescent protein signal gene utilization for flax transformants identification and selection KŮTA, Václav An aim of the thesis was to verify possibilites of in vitro identification and selection of transformed flax tissues and plants based on the fluorescence assessment of objects transformed with a construct bearing the gfp gene coding for green fluorescent protein (GFP). Effect of plant genotype, explant type and modifications of the transformation protocol on fluorescence was evaluated too, as well as the possibility of use for selection of transgenic individuals. Detailed record
	Klastrování genů s podobným regulačním mechanismem během monocytární a garanulocytární diferenciace buněk HL-60 Koutná, I. ; Faltýsková, E. ; Krontorád, P. ; Svoboda, Z. ; Pavlík, T. ; Bártová, Eva ; Kozubek, Michal ; Kozubek, Stanislav In the present study, a high density-microarrays of human cDNA containing 19,000 genetic elements were used to search for differences in gene expression of human promyelic leukaemia cell line HL-60 during monocyte (MD) and granulocyte differentiation (GD). For differentiation to monocytes (granulocytes), the TPA, 12-O-tetradecanoylphorbol-D-acetate (DMSO) were used, respectively. A total number of 4625 (4760) genes were found to be regulated during MD (GD). The genes have been divided into groups according to their kinetics of regulation (R-groups). The genes of the same group are not distributed randomly throughout the genome but form clusters on chromosomes (R-clusters). Thus, functionally interconnected genes are frequently localized near to each other on the DNA molecule. Detailed record
	Analýza genové exprese kolorektálního karcinomu detekované cDNA mikročipy pomocí kombinace různých vyhodnocovacích přístupů Jansová, E. ; Krontorád, P. ; Svoboda, Z. ; Koutná, I. ; Pavlík, T. ; Kozubek, Michal ; Jarošová, M. ; Žaloudík, J. ; Kozubek, Stanislav We have compared colorectal carcinoma tissues with parallel samples of epithelial tissue and identified, by means of Human 1.7K cDNA microarrays, a set of genes with significantly altered expression in 12 patients. We used a combination of several approaches of microarray data analysis to be sure that the results were reliable. Although we have found differences between the results obtained by various image analysis software packages and using different statistical tools, we have identified the group of 22 genes with significantly altered expression in colorectal carcinoma tissue as compared with epithelial tissue using all evaluation approaches. Hierarchical clustering showed the possibility of dividing the patients into two groups according to the presence of metastases in regional lymph nodes. We have proposed 6 genes as potential markers for the detection of the presence of regional metastases in patients. Detailed record

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