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Methodology of ApMV, ACLSV and ASGV diagnostic in apple and pear cultivars and rootstocks by ELISA
Svoboda, Jiří ; Polák, Jaroslav
The presence of Apple mosaic virus (ApMV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) is tested in apple and pear cultivars and rootstocks according to the EPPO recommendation by evaluating symptoms on tree indicators after grafting. This method requires two or three years of planting. For that reason it was prepared and optimised serological testing the presence of ApMV, ACLSV and ASGV by ELISA as a reliable specific methodology. Research results obtained in Crop Research Institute, Prague approve suitability of ELISA for the detection of these viruses in a specific tree tissue during a specific vegetation period. Developed methodological procedures are appropriate especially for state control and supervision laboratories and they are necessary for the certification scheme of fruit trees.
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