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Probiotic foods
Páterová, Alena ; Navrátilová, Jana (referee) ; Vítová, Eva (advisor)
This bachelor thesis summarizes the knowledge about problematic of probiotics and their positive effects on human health. Probiotics are live microorganisms, which, at application in appropriate quantity as component of food, have health benefit in humans and animals. Probiotics are already an important component of treatment in gastroenterology, in hepatology, at treatment of urogenital system, at prevention and therapy of cancer and mycoses. The best known probiotics are lactic acid bacteria, of which most often used are Lactobacillus and Bifidobacterium genera. Lactic acid bacteria because of their unique metabolic characteristics are involved in many fermentation processes of milk, meat and vegetables. We can say, that advances in probiotic bacteria research are beneficial for both the consumers and the producers.
Possibilities of use of food supplements in prevention and health care calves
PÁNIKOVÁ, Monika
The basis for a successful cattle production is the proper care taking of pregnant cows as well as a sufficient care taking of the new-born calves. During the last years, a big attention has been given to this area because the higher farming quality the higher economic benefits and the lower losses in meat and milk yield will be respectively. For this reason, an influence of different feed supplements (Lactovita, Biopolym, Homeopathics) on haematological and biochemical parameters as well as an effect on weight gain and amounts of microorganisms in new-born calves has been studied. The specific aim of this thesis is to evaluate an influence of these supplements on incidences of diarrhea, microbial activity and to assess its effect on selected haematological and biochemical blood parameters. Three experimental and one control group (ten calves per each) were set up in the chosen company. The experimental groups had a modified feeding ratio between the first and the fourth week of life the calves received either colostrum + 5ml "PVB" homeopathic or colostrum + 5 ml "Biopolym" prebiotics or colostrum + 1 pill of "Lactovita", while the control group received colostrum without supplement. The first day after birth, a blood sample and a microbial smear sample were taken. Second sample collection was carried out in the fourth week of life. The calves were weighed every week. Obtained data were processed graphically and in tables and were statistically evaluated. The influence of mentioned feed additives on weight gain and diarrhea occurrence was evaluated from the obtained results. The most of haematological and biochemical parameters have approximately the same values compared to those found by other authors. There were no significant differences in monitored parameters in the experimental groups compared to the control group. Furthermore, no influence was found on blood parameters. Only in case of zinc a significant difference was found between treated groups and control (p=0,0012). Changes between the treated groups (independently on control) were observed in haematocrit, total protein content, cholesterol, zinc and copper. Non-significant differences between the tested groups and control were found in haemoglobin, erythrocytes, leukocytes, glycaemia, urea, LF, GMT, phosphorus, calcium and magnesium. No impact of tested supplements was found on microbial composition. A positive effect of Lactovita, Biopolym and Homeopathic compared to the control group was found in case of weight gain. The obtained results show some positive trends which should be a subject for further research, where total amount of calves per groups is increased to reduce variability.
Influence of selected feeding supplements on the occurrence of coccidias in digestive tract of pheasants
ŠÍP, Pavel
The occurrence of coccidiosis is an enormous problem of pheasant farming. The aim of this study is to show, that this disease can be cured by unconventional dietary supplements. Dietary supplements which were used in this study were as follows: prebiotics (Ascophyllum nodosum), probiotics (Lactobacillus fermentum), homeopathics, Sulfacox and scrap from the seed by Vitis vinifera. The experiment took place in the Ranč Daniela farm in the town Hůrka (close to the Horní Planá Town) from 26.7.2014 to 5.10.2014. Six groups of pheasants were made - three experimental groups and one control group. All groups were consisted of 15 individuals. The pheasant Phasianus colchicus was used in this experiment. Dietary supplements were served for two weeks, than the serving stopped for two weeks and continued again. The samples of excrements were tested all the study long using the floatation method in Sheather's sugar solution. The results were marked and evaluated. The favourable effect of Ascophyllum nodosum, homeopatics and Lactobacillus fermentum on the intestinal tract of pheasant was significant.
Influence of selected feeding supplements on the occurrence of coccidias in digestive tract of chickens
PAZDERKOVÁ, Lenka
Taking preventive measures has a major impact on the overall health status of poultry in every breeding and it can prevent considerable economic losses in this way. The aim of this study was to obtain basic knowledge about the occurence of coccidia oocysts in the faeces and the occurrence of pathological changes in the intestinal mucosa of broiler chickens after the use of selected feed supplements. Experiment was made during winter 2013 and summer 2014 with 250 specimens of one-day broiler chickens. Four experimental groups (prebiotics, probiotics, homeopathy, coccidiostats) and one control group were created. Treated groups were administered by Selected feed supplements were served to chickens during 14 days since the sixth day of their life. Mixed faeces samples were collected twice a week and processed in the laboratory. Also the frequency of coccidia oocysts was monitored under the light microscope. Intestines were collected during the slaughters of chickens (five pieces from every group) and tested to confirm or disprove pathological changes associated with coccidiosis of poultry. Intestines were collected three times after seven days. The Reduced frequency of occurrence of coccidia oocysts in faeces of chickens in all groups of the winter and the summer trial period was demonstrated, while pathological changes of the intestinal mucosa were not proved.
Effect of Lactobacillus casei/paracasei on the immune development of germ-free mice
Kozáková, Hana
Study of the effect of probiotic bacteria Lactobacillus casei/paracasei on the immunological profile of originally germ-free mice. The effect of colonization on the development of allergic sensitization to the main component of birch pollen Bet v 1 was tested.
The basic aspects of nutrition and evaluation of selected haematological and biochemical parameters in the blood in calves
POBORSKÁ, Anna
Healthy and well fed calves are the main prerequisite for high performances. The aim is to evaluate the concept of nutrition of calves and assess the dynamics of selected haematological and biochemical parameters of blood in the sample of agricultural holdings, depending on the influence of selected dietary supplements. The experiment was conducted in the agricultural cooperative Krásna hora nad Vltavou a.s. on the farm Perovice, where they breed Holstein cattle. There were three groups of calves - one control and two experimental. The test groups received dietary supplements to promote active immunity by the instruction. In the first group Lactovita dietary supplements, the latter group food supplement called Biopolym. The first blood sampling was performed from ages 5 to 7 days and a second collection has been made 3 weeks later. Findings and data analysis were summarized and evaluated. The experiment was conducted from August to November 2013.
Utilization of the additives in farm animal nutrition
ADAMOVÁ, Jana
This thesis deals with the use of additives in livestock nutrition, the distribution of these substances as well as their characteristics. The work is focuses on characteristics of a selected group of zootechnical additives. In this group of substances each specific substance added to feed are presented and it also describes their impact and importance in livestock nutrition.
The importance of probiotics and prebiotics in diseases of childhood
JERKOVIČOVÁ, Diana
The major goal of this thesis was to explore and summarize the issue of the importance of prebiotics and probiotics in the prevention and treatment of disease in childhood in the form of theoretical work using a variety of literary sources, both Czech and foreign. The most important prerequisite for proper physical growth, health and psychosocial development of the child is good nutrition. The simplest and most natural way of feeding babies is breast-feeding. Breast milk is absolutely perfect composition, optimum temperature and protects infants against various infections and allergies. The colonization of the intestinal microflora, which affects among other things, our immune and digestive system, begins to occur during childbirth (vaginal and fecal microflora from its mother), and from breast milk or the environment. The settlement is delayed if the child was born by Caesarean section. If infants are only breastfed their intestinal microflora is composed of 90% of healthy bifidobacteria and lactobacilli. Probiotics are referred as living organisms (bacteria and yeasts) which have shown positive influence on the health of the host when administered in adequate amounts. Probiotics in pediatric practice has tripled over the last ten years. The most used are bifidobacteria and lactobacilli. Prebiotics are non-digestible food ingredients that beneficially affect the host by selectively stimulating the growth and / or activity of certain strains of intestinal microflora. The first area of concern of the effect of prebiotics and probiotics in the prevention and treatment of various diseases in children were atopic disease whose prevalence in recent decades, greatly affects the whole society. In case of atopic dermatitis and food allergies there are number of optimistic studies that demonstrate the positive effect of combination of probiotics and prebiotics (synbiotics), on the other hand, there are studies on this issue which are rather skeptical and do not confirm this positive effect. Convincing results come from the area of diarrhoea. Regarding acute diarrhoea that are in children caused mainly by rotavirus, was observed clearly positive effects of probiotics, which occur to shorten the duration of rotavirus diarrhea to about 40 hours, or to reduce the number of stools and the duration of diarrhea by approximately one day, and especially where there has been the early administration. Similarly, in a majority of cases the positive impact of the use of probiotics for diarrhoea associated with antibiotic treatment, especially during simultaneous use. Regarding inflammatory bowel diseases, favorable results are achieved in particular in the case of ulcerative colitis, which is the use of a positive effect of probiotics in the treatment of patients with mild to moderate inflammatory activity comparable to drug treatment, but in Crohn's disease, a positive influence is entirely clear. Positive results also come in necrotizing enterocolitis in preterm neonates. However, these results are difficult to interpret because studies. Very similar results in the effects of probiotics and prebiotics are in the prevention of nosocomial sepsis in preterm infants. Although showing some positive effects, particularly in infants with very low birth weight, there was a positive effect of probiotics or prebiotics in different studies fully demonstrated and it would be desirable to assess the safety and effectiveness of future research. It would be necessary to examine which specific types of probiotics should be used and in what dosage. In general, therefore, could be said that despite all the optimistic results of a positive effect of probiotics and prebiotics in the prevention and treatment of various diseases, there is the need for further research, that this positive effect is confirmed or refuted.
Influence of selected feeding supplements on the occurrence of coccidias in digestive tract of pheasant
ŠÍP, Pavel
The occurrence of coccidiosis is an enormous problem of pheasant farming. The aim of this study is to show, that this disease can be cured by unconventional dietary supplements. Dietary supplements which were used in this study were as follows: prebiotics (Biopolym), probiotics (Lactovita containing bacteria Lactobacillus sporogenes) and homeopathics. The experiment took place in the Ranč Daniela farm in the town Hůrka (close to the Horní Planá Town) from 1.7.2012 to 18.11.2012. Four groups of pheasants were made - three experimental groups and one control group. All groups were consisted of 13 individuals. The pheasant Phasianus colchicus was used in this experiment. Dietary supplements were served for two weeks, than the serving stopped for two weeks and continued again. The samples of excrements were tested all the study long using the floatation method in Sheather's sugar solution. The results were marked and evaluated. The favourable effect of Biopolym and homeopatics on the intestinal tract of pheasant was significant.
Functional foods in human nutrition
ZEVLOVÁ, Veronika
Bachelor thesis deals with functional food and their role in human nutrition. The history of functional food, their characteristics, components, evaluation of individual species and the impact on the health of the consumer is described here. A lot of researches are focused on the topic of functional food, but no legally definition have been established yet. Even in the Czech Republic or the EU. Only the certain designations are used, which have a small deviations in different countries. Functional foods can be formed in different ways. It´s the elimination of certain harmful substances, a modification, but the most commonly used method is to add some substance to food. These substances may have various origins. The most commonly used are probiotics and prebiotics, antioxidants, anticarcinogens, fiber and many more. As the types of functional food there are mentioned cereals and products made from them, as well as meat and meat products, fruit and vegetables, vegetable fats, foods from stevia and very important probiotic food. Then the study focuses on the behavior of these types of food in prevention of civilization diseases, namely diabetes, obesity, hypertension, non-alcoholic fatty liver disease and the Alzheimer´s disease. Subsequently, this section deals with the importance of functional food for particular population groups. Attention is given to children, the elderly, pregnant women and athletes.

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