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Characteristics of crude protein fractions in ruminant nutrition
KOUKOLOVÁ, Marie
Physiology of nutrition and feeding of farm animals is an essential factor influencing production efficiency and its quality. For these factors is important to provide optimal supply of nutrients by suitable feeds. Felicitous feed ration raise the economic realization of genetic potential of animals. Therefore we have to know the nutritional requirements of animals, including microorganisms, whose function in the diet of cattle is irreplaceable. The aim of this study was to determine the nutritional value of forage and lupine feeds (n = 8) by laboratory methods. Samples were analyzed for individual feed nutrients (crude protein (CP), fat, crude fiber, ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL)), gross energy and nitrogen fractions, and the results were statistically processed. Determination of individual nitrogen fractions was according to methods of Licitra et al. (1996). For this purpose were used following laboratory methods: (1) determination of non-protein nitrogen (NPN) (2), determination of soluble nitrogen and protein, (3) determination of nitrogen insoluble in acid detergent (ADIP), (4) determination of nitrogen insoluble in neutral detergent (NDIP). From these were obtained the results of NPN, IP (insoluble protein), SOLP (soluble protein), ADIP (nitrogen insoluble in acid detergent) and NDIP (insoluble nitrogen in neutral detergent). Averaged values (g.kg-1 of dry matter) of were 144.3 for NPN, 97.5 for IP, 78.7 for SOLP, 30.4 for ADIP and 80.3 for NDIP. Nitrogen fractions, i.e. fraction A (nonprotein nitrogen), fraction B1 (rapidly degraded protein), fraction B2 (intermediately degraded protein), fraction B3 (slowly degraded protein) and fraction C (bound protein) were calculated by equations of Ghoorchi and Arbabi (2010). The range of values was from 2.5 to 51.2 % of CP for fraction A, from 13.4 to 20.7 % of CP for fraction B1, from 0 to 19.9 % of CP for fraction B2, from 5.5 to 51.9 % of CP for fraction B3 and from 10.1 to 32.4 % of CP for fraction C.
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