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Analysis of ketoprofen, methylparaben and propylparaben by micellar electrokinetic chromatography
Balcar, Josef ; Pospíšilová, Marie (advisor) ; Polášek, Miroslav (referee)
1. ABSTRACT ANALYSIS OF KETOPROFEN, METHYLPARABEN AND PROPYLPARABEN BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY Josef Balcar Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, Heyrovského 1203, Hradec Králové In this study, a new micellar electrokinetic chromatography (MEKC) has been developed for the simultaneous analysis of ketoprofen, methylparaben and propylparaben. Separations were carried out in a fused silica capillary (50 cm × 75 μm i.d.) at 30 kV with UV detection at 200 nm. The optimal background electrolyte was 50 mM tricine buffer (pH* 8.3) containing 30 mM sodium dodecyl sulfate as a surfactant and 15 % (V/V) methanol. Rectilinear calibration ranges were 10.0 - 200 mg/100ml for ketoprofen, 0.2 - 4.0 mg/100ml for methylparaben and 0.1 - 2.0 mg/100ml for propylparaben. The MEKC method was applied to the determination of the analytes in pharmaceutical preparation. The total analysis time was <13 min.
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