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National Repository of Grey Literature 12 records found  previous11 - 12  jump to record: Search took 0.01 seconds. 
Factors regulating the expression and activity of digestive enzymes in the tick \kur{Ixodes ricinus}
 KONVIČKOVÁ, Jitka
The intracellular proteolysis of ingested meal plays an essential role in tick development. The thesis focuses on the factors influencing the expressions and activities of digestive enzymes in Ixodes ricinus females during the feeding and post-feeding period. We have revealed the effect of fertilization on blood feeding and digestion. The females cannot reach the rapid engorgement phase without being fertilized. The rate of mated females in the nature proved the presumption that mating can occur even off the host. Implementation of in vitro feeding technique further extended our current knowledge about tick digestive apparatus. Adult females were fed on hemoglobin-rich and hemoglobin-poor diet and the mRNA expression levels of digestive proteases were determined. In line with obtained data, we assumed that albuminolysis is conducted by the same or similar pathway as hemoglobinolysis. The gene silencing method and protein immuno-detection were used to unequivocally identify the isoforms of 'early expressed' IrCL1 and 'late expressed' IrCL3 isoform of cathepsin L.
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Physiological mechanisms of aging in maless of model insect species
 PROVAZNÍK, Jan
Trade-off between reproduction and longevity is a widely accepted fact, yet proximate mechanisms are scarcely understood. In this work I tested differences in lifespan between diapause and non-diapause males of a model insect, the linden bug Pyrrhocoris apterus. Also the role of juvenile hormone in regulation of longevity and immunity (measured by relative phenoloxidase activity) was assessed. In addition to that, I examined if juvenile hormone is the mediator of reduction in longevity induced by mating.
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National Repository of Grey Literature : 12 records found   previous11 - 12  jump to record:
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