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Basic haematological parameters and their influencing by pollutants in common carp a review
POLANSKÁ, Daniela
The objective of this study was to describe the methods of determining the main haematological parameters and obtain as much information as possible about pollutants that may influence them. The subject of this thesis is the common carp (Cyprinus carpio L.). As far as pollutants are concerned, I focused on nitrogen compounds (ammonia, nitrite and cyanide), metals (Zn, Cu, Fe), heavy metals (Hg, Cd, Pb, Cr), petroleum substances, pesticides (organophosphates, triazines and diazines, pyrethroids), pharmaceuticals and natural supplements and persistent organic pollutants. Collected information imply that heavy metals and pesticides have the most important effect on haematological parameters of the common carp. Heavy metals cause changes in haematological parameters and cell anomalies and pesticides may cause haematopoietic failure, which especially means a decrease of number of red blood cells, the concentration of haemoglobin, the haematocrit value and the changes in the presence of different leukocyte forms. This study summarizes the knowledge of the effects of the aforementioned pollutants and highlights the danger of their effect as well as the possible threat to fish health, not just of the common carp. The information dealing with the effects of many pollutants on the blood parameters of fish is missing in available scientific literature.
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Vliv syntetických progestinů na vývoj gonád kapra obecného
PROFANT, Vít
This paper was focused on effect of synthetic progestins on gonadal development in fish. A 160-day chronic test on common carp (Cyprinus carpio), was run underlaboratory conditions. The fish was divided into five experimental groups. The first control group included clear diluted water without any occurrence of progestins. The second control group was kept in diluted water with dissolving agent DMSO (dimethylsulfoxide). The third group of fish was exposed to gestogene at a concentration level of 2 ng.l-1 and the fourth group was exposed to drospirenone at a concentration level of 2 ng.l-1. The last group (Mix) was exposed to gestogene and drospirenone simultaneously, where both substances corresponded to the concentration of 2 ng.l-1. The experimental groups were dosed with progestins dissolved in DMSO, since they have lipophilic nature. The determination of the influence of synthetic progestins on the gonadal development was based on histological examination of the exposed fish samples in toto. There was any signifficant difference in fish sex ratio and in percentage of intersex occurrence between the groups that wwere exposed to individual synthetic progestins and the control groups. However, the exposure of the mixture of drospirenone and gestogene (Mix) showed a significant difference. Primarily, a high percentage of intersex was detected with the number reaching 36.6 %. The occurrence of intersex suggested a twist in sex of exposed fish through ontogenesis.
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Demembranation of fish sperm: Design and verification this procedure for the different species of freshwaterfish and demonstration usage of this technique by study the effect of heavy metals to sperm axoneme
BLAŽKOVÁ, Jaroslava
The object of this study is to design demembranation method on four freshwater species and its application on study of the influence of HgCl2 on the axoneme and motity sperm motility parameters. Demembranation was designed and examined for all investigated species common carp (Cyprinus carpio), sterlet (Acipenser ruthenus), perch (Perca fluviatilis) and african catfish (Clarias gariepinus). One-step and two-step method was designed and tested for common carp. One-step method was designed for sterlet and perch. Two-step method of demembranation was designed for african catfish. Demembranation was designed and examined for all species under examination. Sperm motility was evidently increased above normal physiological value. Other sperm motility parameters (velocity, percent of motile cells) slightly decreased. HgCl2 in concentration 0,01 mM to the demembranation medium didn't show effect on flagellar microtubule aparat and then to the motility parameters, except sterlet; demembranated sterlet sperm was inhibited at all used concentration of HgCl2. Concentration 0,1 mM had inhibition effect on carp and africant catfish spermatozoa. Concentration 1 mM HgCl2 inhibited sperm of all tested species.
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Fish excretion of ammonia under different oxygen environmental conditions
MÜLLEROVÁ, Lucie
The aim of this work was to find out and compare amount of ammonia nitrogen excreted by fish in different oxygen saturation of water. Experiments (preliminary, main and additional) were made with ornamental form of common carp (Cyprinus carpio f. Koi) at Laboratory of Aquatic Toxicology and Ichtyopathology RIFCH in Vodňany. The preliminary (orientation) experiment was made in three different dissolved oxygen concentrations (I. 0,65 - 2,66; II. 2,43 - 5,70; III. 6,40 - 6,50 mg.l-1 O2) and fish production of ammonia nitrogen after 2 hours was I. 24,61; II. 85,29; III. 70,19 mg N-NH4+ per 1 kg of fish weight. The fish were also exposed to different oxygen conditions (hypoxia0,76 - 1,46; normoxia 9,8 - 9,46 mg.l-1 O2) in the main experiment which was repeated six times. Detected production of ammonia nitrogen in recalculated to 1 kg fish weight was 25,01 +- 3,14 mg N-NH4+ by the fish in hypoxia and 32,56 +- 4,08 mg N-NH4+ by the fish in normoxia after two hours of experiment duration. Detected difference was statistically significant (P < 0,05). Haematology and biochemical blood examination of fish which were exposed to hypoxia proved statistically significant increasing number of leukocytes, value of haematocrit, concentration of ammonia and glucose was made in the end of experiment. The additional experiment was repeated five times, the fish were exposed to hypoxia there (0,99 - 1,21 mg.l-1 O2) and the control fish were kept in water with concentration of dissolved oxygen between 7,67 - 9,11 mg.l-1. Statistically significant differences in ammonia nitrogen production were already found out in these groups of fish after two hours of experiment duration and difference increased markedly after 4,5 hours (fish production of ammonia exposed to hypoxia 47,92 +- 4,91 mg.kg-1 and in the control fish 101,53 +- 8,31 mg.kg-1). Production of ammonia by the fish originally exposed to hypoxia almost aligned to production of control fish during 2,5 hours after an aeration had been added to the fish in hypoxia. Production of ammonia nitrogen was 93,38 +- 17,82 mg.kg-1 by the fish originally exposed to hypoxia and 110,13 +- 18,21 mg.kg-1 by the control fish. This difference was not statistically significant yet. The results of experiment proved that fish ability to excrete ammonia from body is closely connected with oxygen conditions in water environment.
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The phosphorus balance in semi-intensive rearing of carp
ŠIMÁNEK, Josef
The aims of this bachelor thesis are (i) to verify the generally recognized fact that supplementary feeding has an effect on the increase in phosphorus concentration in the surface water; and (ii) to explore a potential reduction of wastes by supplementary feeding with thermally treated cereals. The experiment was run in two consecutive growing seasons in the years 2012 and 2013. Four ponds were selected for this study. Cereals were applied as supplementary feed in three ponds: In two of them, thermally treated cereals were applied and in the other one, untreated cereal grains were applied. The efficiency of these feeds was compared with the results from the control pond, where fish stock was not supplementary fed. The results showed that bioturbation had a bigger effect on water quality than the input of nutrients from feeds, since water transparency parameters were statistically different (P < 0.05; in the control treatment compared to the ponds that were supplementary fed (higher value of turbidity and suspended solids, lower water transparency). No significant differences were observed in zoobenthos abundance, although the lowest density was in the control pond. However, no differences in zooplankton density between the control ponds and the supplementary fed ponds were found. On the other hand, differences in productive efficiency (SGR) of both types of feeds were found, which was highest in thermally treated ponds. The fact that the amount of phosphorus depends on the age of fish and bioavailability of phosphorus in feed was confirmed. Thermally treated cereals are economically inexpensive and the use of thermally treated cereals may provide a framework for the sustainable management of carp ponds, resulting in improved phosphorus budget over the entire pond system.
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Cryopreservation of common carp (\kur{Cyprinus carpio} L.) sperm under different freezing conditions
SOCHOROVÁ, Denisa
In the present study, we examined several cryoextenders previously used by several authors and various freezing protocols to determine the relative importance of each parameter on sperm freezing. The effects of controlled seeding and changes in cooling rate at different stages of freezing were also examined. Sperm samples from seven individual carp males were frozen in 0.5 ml straws by conventional freezing. Cooling rates were determined by monitoring the sample's internal temperature. We compared four freezing protocols, which involved placing sperm samples at various levels (1, 3, 6, and 9 cm) above the liquid nitrogen (LN) surface (corresponding to -190, -150, -110, and -70 °C, respectively) for 20 min followed by transferring the samples into LN. Freezing at 3 cm above the LN surface resulted in the highest motility (33 ? 8 %) and velocity (118 ? 9 ?m/s) of spermatozoa after thawing and diluting in swimming medium. We determined that -90 °C is an optimal temperature at which immersing the samples in LN does not affect sperm motility after thawing. The sperm motility of samples immersed in LN before or immediately after the crystallisation point (-16 °C) was 0 %. Motility of spermatozoa cryopreserved with or without a seeding procedure was not significantly different after thawing. Therefore, we hypothesise that supercooling the sample during the conventional freezing procedure is not the main damaging factor during carp spermatozoa cryopreservation.
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The influence of fishery farming intensity on water quality in ponds
KREJČÍ, Filip
The introductory part deals with the history of czech fish farming and there is given further information on selected quality parameters of pond water, which are important and risky in terms of fishery production. The main part of the thesis summarises data from the literature, final reports and RIFCH (The research institute of fish culture and hydrobiology) Vodňany database is processed in a form of tables and graphs for clear presenting of physiochemical properties of pond water, including brief details about the way of fish farming, eventually about other important factors, which could also affect the water quality. The main data source of my thesis were four projects, which dealt with this issue during a relatively long period of time, from the seventies of the last century until the year 2012. The greatest attention was paid to data gained under NAZV QH-82113 project "Careful and effective fish farming with maximal utilization of current trophic potential and keeping of sufficient water quality and fish production" in the years 2008-2012 at experipentals ponds of RIFCH Vodňany. There was a comprehensive set of data gained from semi-intensive conditions, where working multiple times and in a very well comparable and under well documented conditions (size of ponds, water supply, fish farming methods) was possible. Based on some published data and data obtained from VÚRH Vodňany database, it was able to demonstrate a direct influence of the size of initial stocking density on the water quality of ponds (especially the concentration of suspended solids, volume of organic pollutions and the total concentration of a phosphor). Generally, parameters, which often do not comply with legislative requirements, could be described as suspended solids, concentration of oxygen, ammonium, total phosphor and Biochemical oxygen demant (BOD5) and Chemical oxygen demant (CODMn or CODCr) values, whose values are sometimes influenced by factors such as for instance the history of a pond, other than production usage of the pond in the previous period (deponie of waste from livestock production, recreational activities etc.)
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The effect of supplementary feeding of market carp by cereal feeds on water quality
STROUHAL, Jiří
The purpose of this thesis was monitoring the impact of supplementary feeding to selected parameters of water quality (temperature, oxygen, pH, alkalinity, conductivity, turbidity, total suspended solids, chlorophyll a, CODCr, BOD5, TP, P PO43-, TN, N NH4+, N NO3). This experiment took place in storage ponds in Třeboň for 120 days. For the supplementary feeding were used nonmodified wheat, thermally wheat and thermally and pressed wheat. There were chosen 2 storage ponds for every group. Fish were fed 3 times a week. For comparison of results it was deployed a.k.a control where fish were dependent on natural food. It was taken total 12 samples in which were determined chemical and physical properties of water. Only one statistic (p < 0,05) difference was recorded in concentration of oxygen in storage ponds, where fish were fed by thermally and pressed wheat (5,79 + - 1,38 mg.l-1) in comparison with control (7,00 + - 1,73 mg.l-1). Other parameters did not exhibit significant changes among the monitored groups (p > 0,05).
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Susceptibility of Individual Fish Species to Koi Herpes Viral Disease - a research study
KOSTLÁN, Jakub
Koi herpes viral disease (hereinafter referred to as "KHVD") is a dangerous viral disease, outbreak of which was recorded in Israel in 1998 for the first time. Due to exhibitions and international fish trade it spread almost to the whole world in the course of the next 17 years and it caused a loss of many tonnes of both breed and consumption carp as well as it inflicted significant economic losses calculated in the tens millions of dollars. The European Union has added the koi herpes viral disease, due to its hazards, to the list of non-exotic diseases. Since 2008 there is a duty to report this infection in the Czech Republic as well. Koi herpes virus classified in the system of viruses as Cyprinid herpesvirus 3 (CyHV-3) is considered the infective agent. Koi carp (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio) are considered the main species susceptible to KHVD. However, it has been proved that the virus may be also detected in tissues of other fish species and in some of them clinical symptoms may appear. It is of high degree of probability that such fish species may play the role of viral communicants and this presumption has already been proved in some species in experimental conditions. With a view to the fact that koi herpes viral disease is incurable and application of a vaccine is impossible in the Czech Republic, for protection of fish breeding it is crucial to prevent bringing the disease in the territory. Mass fish perish caused by KHVD shall be prevented by strict inspection processes of the imported and exported fish, monitoring of the disease spread and responsible breeders' approach. The major objective of this work was to summarise available information about koi herpes viral disease and susceptibility of the individual fish species to this dangerous viral infectious disease in a form of a research study.
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Growth of tagged common carp (Cyprius carpio) in selected water reservoirs, Morava River drainage area
Prokeš, Miroslav ; Baruš, Vlastimil ; Habán, V. ; Mareš, J. ; Peňáz, Milan ; Halačka, Karel ; Krejčí, R. ; Vetešník, Lukáš
The project monitoring the growth of tagged two-season-old common carp (K2) being stocked into the selected reservoirs managed by the Moravian Angler Association was initiated in 2001 in the reservoir of Nové Mlýny. Few other reservoirs, namely the Dalešická, Brněnská, Vranovská and Letovická, were then also studied in course of the following period up today. The altitude of investigated reservoirs ranges 171-382 m a.s.l., the flooded area of water bodies 104-1600 hectares. The stocking series in every of reservoirs were performed in the course of subsequent five years and two thousands of tagged carps (K2) were always yearly stocked in every of reservoirs. In total, 50 335 (17.61 inds per hectare) tagged carps have been stocked and 1361 individuals (0.48 inds per hectare) were recaptured and reported by the anglers. The mean recapture rate of tagged fish reached thus 2.70 % (1.02 - 4.74 %). A significant negative relationship was also ascertained of altitude (in m a.s.l.) and growth indices. The maximum growth rate was found in the Novomlýnská reservoir (mean annual weight increment 1725 g). Gradually lower weight increments were found out in the Vranovská reservoir (1064 - 1200 g), Dalešická R. (1050 g), Brněnská R. (1070 g) and Letovická R. (630 g). A similar sequence existed also in growth of other parameters studied, i.e. total length (TL), specific growth rate (SGR), and weight condition expressed by means of the Fulton condition factor (FCF). On the basis of these results, some new, more convenient, rules were suggested for stocking of common carp.
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