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Distribution, ecology and conservation of critically endangered Alcon Blue (Phengaris alcon f. alcon) in the Czech Republic
Kupková, Barbora ; Sedláček, Ondřej (advisor) ; Pech, Pavel (referee)
The state of the populations of many insects is critical in the Czech Republic, other species have already extinct. Among the species balancing at the limit of survival is myrmecophilus Alcon Blue (Phengaris alcon), especially its ecological form (P. alcon f. alcon), which is associated with the Marsh Gentian (Gentiana pneumonanthe) during the early larval stages. The general aim of my work was to evaluate the current distribution of the Alcon Blue butterfly (P. alcon f. alcon) in the Czech Republic, to estimate the size of the remaining populations and to propose a practical action plan for conservation of the species in the Czech Republic. The analysis of the databases showed that the species survives theoretically in 11 localities. The field survey in 2016 revealed, however, that at five other sites a species in the recent past was extinct. The remaining 6 localities are concentrated in Southwestern Bohemia on a total area of only 20 hectares. Two population is however close to extinction. The cause of local extinction of Alcon Blues was the extinction of host plants. The main factors influencing the size of butterfly populations in the last localities were the abundace and vitality of host plants (number of fertile shoots, height). Part of my work was an attempt to reintroduce Alcon Blue to a...
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Analýza karyotypu vakonošů (Psychidae, Lepidoptera) metodami klasické a molekulární cytogenetiky
HEJNÍČKOVÁ, Martina
Due to their phylogenetic position, Psychidae play an important role in the investigation of the W chromosome origin in Lepidoptera. Several species of Psychidae were tested for the presence of sex-chromatin and investigated via comparative genomic hybridization. Furthermore, odd chromosome numbers and a Z univalent were observed in females. Overall, this study brings tangible evidence for the absence of the W chromosome in Psychidae, thus contributes to complex knowledge of the W chromosome evolution. In addition, karyotypes of the given species were analyzed using 18S rDNA and histone H3 probes. The results indicate relative stability of their karyotypes.
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Population genetics and phylogeography of a vanishing species: the case of \kur{Argynnis niobe}
LEŠTINA, Dan
This study evaluates the state of the Niobe fritillary (Argynnis niobe) in Europe, both in terms of (a) current knowledge of its distributional changes and of (b) the genetics of the surviving populations. It uses mitochondrial sequences and microsatellite markers to assess the phylogeographic history of the species, and the impact of the multiple local extinctions at the same time. The data are evaluated by a standard set of methods genetics and phylogeography population and a brief synthesis including recommendations for conservation management is given.
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Synantropis lepidopterans in Pelhřimov cemeteries
RŮŽIČKA, Libor
The presented study is to a degree a continuation to the author's bachelor thesis: Occurrence of Lepidoptera order within the district of Pelhrimov. This study is now focusing on the evaluation of research on moths found in five cemeteries located within this district during the year 2014. The area of interest is located in the south of the Czech republic, in the Region Vysocina. Measured from an entomological point of view, it is a cooler region with a lower diversity of the Lepidoptera order. Cemeteries in general represent very interesting localities well suited for entomological research, which has not been so far sufficiently recognized by members of the entomological academic community, but may represent a refugium in otherwise uniform landscape. During my research I recorded altogether 130 moth species during the period in question. The location number 5 (Cemetery in Cetoraz) has been evaluated as the one with the highest species richness, reaching documented occurrence of 63 species of the order Lepidoptera.
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Phylogeography of \kur{Boloria eunomia} and comparison with similarly distributed species
MAREŠOVÁ, Jana
Phylogeography, phylogeny and population structure were studied in two similarly distributed butterflies, the Bog Fritillary (Boloria eunomia) and the Small Pearl-bordered Fritillary (Boloria selene). Sequences of mitochondrial gen for COI and nuclear Wingless and Arginine Kinase were used for analyses, for reconstruction of evolutionary relationships within both species and for comparison of phylogeography patterns.
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Phylogeography of mountain butterfly ??Bicyclus anisops in West Africa
PETRŮ, Vojtěch
The Gulf of Guinea Highlands is a unique mountane range of volcanic origin, situated on the African mainland in the borther of Nigeria and Cameroon, and the Gulf of Guinea islands of Bioko, S?o Tomé and Príncipe. This area is highly unique as the only large mountains in central and western Africa. The high degree of isolation of these mountains generated high degree of endemism of many groups of organisms; the whole region is thus one of the world biodiversity hotspots (known as the West African Forests). Despite its tremnedous conservation importance, this mountain range is under strong anthropogenic influence, as one of the most densely populated areas in tropical Africa. Despite all these factors, the conservation efforts in West Africa focused mainly on lowland areas of rain forests, whilst only few conservation effort is focused to the mountain habitats. As the other result, the lack of comprehensive biogeographic and phytogeographical studies of mountain flora and fauna is obvious. In this work, I have summarized the results of all crucial studies of the biogeography of the Gulf of Guinea Highlands. I also obtained sequences of three mitochondrial genes (COI, COII, and ND1) of an endemic montane butterfly Bicyclus anisops. Variability in the studied genes was relatively low. Phylogenetic analyses separated haplotypes into 7 well-established groups (1st Mt. Cameroon, 2nd Nkogam I, 3rd Nkogam II, 4th Bakossi-Kupe-Manengouba-Bani, 5th Mbam, 7th Hosséré Enla Fabo 6th all others localities (tj. Bamenda-Banso Mts., Acha Tugi, Mbam, Ngel Nyaki, Gotel Mts.)) but did not significantly resolved relationships among them. Based on the hyplotype diversity of B. anisops as an umbrella species, Mt. Cameroon, Nkogam, Mbam Massif, Mbiame, Hosséré Enla Fabo, Mt. Manengouba, Mt. Kupe and Mt. Oku are considered as localities of the highest conservation importance.
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Ecology and evolution of mountain butterflies
KLEČKOVÁ, Irena
The thesis deals with speciation processes, thermal ecology and habitat use in Holarctic mountain and arctic butterflies. It demonstrates a crucial role of environmental heterogeneity for speciation, survival of butterfly lineages, coexistence of closely related species and, finally, for resource use of sexes with different habitats demands at the level of individual species.
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