National Repository of Grey Literature 13 records found  previous11 - 13  jump to record: Search took 0.00 seconds. 
The Study of the genetic variability of phytoplasmas
ROHÁČKOVÁ, Helena
Phytoplasmas are bacterial intracellular plant pathogens that cause devasting yield losses in diverse crops worldwide. Phytoplasmas were detected in clover and Catharanthus roseus plants, pear, apple and apricot trees. SecA and 16S rRNA genes, spacer region and 23S rRNA gene of five phytoplasma isolates were sequenced.
Stady of apple proliferation phytoplasma diversity in the Czech Republic.
STREJČKOVÁ, Monika
Phytoplasmas are prokaryote organisms of the Mollicutes class. So far they have been described on more than 800 plant species. Apple proliferation phytoplasma, which classifies in the ribosomal subgroup 16Sr X-A (Apple proliferation, AP, ?Candidatus Phytoplasma mali?), belongs amongst the agriculturally most important phytoplasmas. AP is considered a quarantine organism in Europe and in North America. Common symptoms caused by AP are as follows: notably enlarged stipules, which are morphologically like real leaves ? they have petiole and lamina; the edge of the stipules is irregularly dentate small fruits with fewer seeds and reduced flavour quality premature reddening of leaves, witches' broom appearance, abnormal growth of shoots in the autumn, repeated blossoming DNA isolated from trees suspected to have AP present was tested in the presented thesis. The trees originated from the gene pool, mass production planting as well as from planting of small producers. To detect phytoplasma, the polymerase chain reaction (PCR) was used with the subsequent analysis of restriction fragment length polymorphism (RFLP). To detect and differentiate AP phytoplasma subtypes, direct PCR with primers rpAP15f/rpAP15r amplifying the DNA of ribosomal proteins rpS3 and rpl 22 with subsequent restriction breakdown by AluI enzyme.
Detection and identification of phytoplasmas infecting pear trees
ROHÁČKOVÁ, Helena
Phytoplasmas are bacterial intracellular plant pathogens that can cause devasting yield losses in diverse crops worldwide. Phytoplasmas were detected using molecular methods in pear trees. RFLP analysis revealed the presence of pear decline, apple proliferation, clover phyllody and stolbur phytoplasmas in examined samples singly or in mixed infection.

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