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Evaluation of cultivar effect on tuber yield structure and selected parameters of tubers in Jerusalem Artichoke (\kur{Helianthus tuberosus} L.)
VANĚČEK, Petr
The aim of this bachelor's thesis was the evaluation of the effect of the Jerusalem artichoke´s (Helianthus tuberosus L.) genotype (clone) on yield structure and selected parameters of the tubers. A one-year field experiment was established on 25th April 2012 on the experimental station of the University of South Bohemia in the České Budějovice (altitude 395 m, a cereal production area), where 8 different genotypes (Refla, Lola, Gigant, Karina, Völkenroder Spindel, Úrodný, Běloslupký, C 63) were planted in 3 replications (spacing at 0.3 x 0.75 m). Úrodný and Gigant were evaluated as the earliest clones (appearing of the flower head 84 days after the date of the planting). The tubers of 5 consecutive plants from the middle row in each replication were harvested in autumn (on 31st October). All clones except clone Běloslupký (due to late planting) were statistically evaluated. The yield values moved in the range of 33.48 t/ha (C 63) to 44.74 t/ha (Úrodný). Genotype Völkenroder Spindel produced the most of the tubers per plant (average 24.3), but we identified the smallest average weight of the tubers (34.73 g) at this clone (R = 0.84%). The least tubers were produced by clone C 63 (16.3). The highest average weight of the tubers was achieved by genotype Úrodný (73.59 g; the 2nd place belongs to clone C 63 55.63 g). We used the method of the lyophilization (Alpha 1 4 LSC) to determine the dry matter content in tubers. The values of the dry matter content ranged from 18.85 % (Lola) to 22.36 % (Völkenroder Spindel). The quantity of the proteins and nitrogen contained in the dry matter were other parameters which we analyzed (BCA Protein Assay Kit, Pierce). The highest values of the proteins contained in the dry matter were obtained at the genotype Refla (6.13 %) and the lowest at the genotype Úrodný (3.90 %). The values of the nitrogen content moved in the range of 0.68 % (Lola) to 1.02 % (C 63). When we used methods of the SDS PAGE and the automatic chip electrophoresis we determined only quantitative differences of the proteins composition in the tubers. The approximate molecular weights of the identified proteins were 8, 20 30, 35, 40, 50, 65, 80, 85 a 90 kDa.
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Present possibilities of protein concentrates production from plant sources
KRISTINOVÁ, Helena
The aim of the bachelor thesis was gather information about present possibilities of proteins concentrates production from plant sources. I focused on dried pea (Pisum sativum L.), soybean (Glycine soja) and potato tuber (Solanum tuberosum L.). The protein concentrates from leguminous plants are produced by three basic processes, i.e. acid leasing (at ~pH 4.5), extracting with aqueous alcohol (60 - 90%), and denaturing the protein with moist heat before extraction with water. Pea protein is prepared from pulverized beans or shelled peas by dry or wet treatment. The acquired protein is used in food and feed industry. Soy protein concentrates are prepared from dehulled and defatted soybeans by removing most of the water-soluble, nonprotein constituents. Soy proteins are mostly used in the bakery, in the meat processing industry, in the production of dairy products and healthy food products. Proteins are used in many food products as emulsifiers. The most potatoes are grown for direct consumption, for processing into the food products and for processing of starch is due to the allocated and restrictive quotas, a smaller proportion of cultivated potatoes. In the production of the starch arise a side product PFJ (potato fruit juice). In order to isolate proteins from potato fruit juice appears to be most efficient heat coagulation of steam injection, followed by separation and precipitation of proteins and their preservation by drying. This provides a final product which is offered as a protein feed for domestic and farm animals. Emulsifying properties of undenatured potato proteins have been studied quite intensively, only industrial production of denatured proteins is limited for the one producer in the EU.
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Noninvasive treatment of tinnitus
AMBROŽOVÁ, Anna
Bachelor thesis "Noninvasive treatment of tinnitus".Tinnitus can be perceived in one or both ears or in the head. It is usually described as a ringing noise, but in some patients it takes the form of a high pitched whining, buzzing, hissing, humming, or whistling sound. Subject may hear a sound, such as a ringing or roaring, that does not come from his/her surroundings (nobody else can hear it). (13)The main target of this work is the estimation of the efficiency of the treatment by electro-stimulative instrument Ti - ex (combined treatment) and its comparison with infusion treatment.
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