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Antimicrobial activity of bacteria isolated from Pectinatella magnifica
Holečková, Karolína ; Vlková, Eva (advisor) ; Geigerová, Martina (referee)
The thesis is comprised of two parts. The first part is dedicated to the literature search, overview, which is focuses on the summary of information that was previously found not only about the life and reproduction of freshwater bryozoan Pectinatella magnifica, but also the importance of marine species bryozoans. Furthermore, importance of antimicrobial active substances and most commonly used laboratory methods for determination of microbial activity are shown in the report characteristics. The aim of the review was to summarize the most important facts underlying the experimental hypothesis described in the second part of this work. The reason for testing the antimicrobial activity of symbiotic bacteria isolated from Pectinatella magnifica is that antimicrobial activity of symbiotic bacteria and also bryozoans´ extracts were demonstrated in the marine bryozoans. Because of this, deeper knowledge of these largely unknown animals has become of high importance. Bacteria isolated from Pectinatella magnifica colonies collected in 2012 and 2013 in South Bohemia were tested for their antimicrobial aktivity against many strains of bacteria. Diffusion method was chosen for testing of the antimicrobial activity. Instead of application by paper disks, method using holes into solid agar was used. Into these holes, precisely selected volume of the supernatant of bacterial isolates has been dosed. In total, 170 supernatants were tested against 80 strains of bacteria. In the case of supernatant containing antimicrobial substances produced by the tested bacteria, an inhibition zone around the hole would be created. The antimicrobial activity has been confirmed in three cases while testing supernatant of symbiotic bacteria Aeromonas veronii and Pseudomonas moraviensis against strains of clostridia. Furthermore, it has also been confirmed, that Aeromonas veronii inhibited the growth of Pseudomonas moraviensis. In the remaining studied isolates, antimicrobial activity suppressing the growth of test bacteria has not been confirmed.
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