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Forenzně významní zástupci řádu brouci (Coleoptera) vyskytující se na území České republiky
Wrzecionková, Nicole Elizabeth
The process of gradual settlement of the dead body by necrophagous insects plays an important role in forensic practice, especially when determining the time of death. This work deals with the decomposition of dead body of vertebrates, forensically important representatives from the order of Coleoptera in the Czech Republic. Experimental objects for our purposes were domestic pig and chicken. The research was realized from December 2017 to March 2019. During the study samples of larvae and adults were collected from the dead bodies and subsequently identified by anatomical-morphological and molecular-genetic methods. These methods were compared with each other. For research purposes was necessary to isolate the mitochondrial DNA, amplify the fragment of cytochrom c oxidase I (COI) gene by the PCR method and then sequencing it mitochondrial DNA. The BLAST and BOLD databases showed the same outputs and based on the results, the individuals were classified into individual families of the Coleoptera order.

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