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Identifikace alel pro zásobní proteiny obilky tritikale pomocí DNA markerů
Svojsíková, Nikola
Triticale belongs to the amphiploid cereals and was derived by crossing wheat and rye. Its baking quality is substantially worse than in wheat. The baking quality is determined by a composition and a content of the storage proteins. Both high and low molecular glutenin subunits have a major effect on the final quality of dough. The secalins of rye belong amongst the storage proteins of triticale, which negatively influence the baking quality. These are the reasons why lineages having translocated chromosome 1R and containing subunit Glu-D1d, which positively influences the baking quality, were created. The thesis is focused on identification of the allelic composition of loci of high molecular glutenin subunits (HMW-GS), low molecular glutenin subunits (LMW-GS), loci Pina a Pinb and null alleles of Waxy genes. 23 selected genotypes of triticale were analysed by using DNA markers based on polymerase chain reaction (PCR). Allelic composition of loci HMW-GS (Glu-A1, Glu-B1, Glu-D1), LMW-GS (Glu-A3) and Pina-D1, Pinb-D1 was described and null alleles were detected in the loci Wx-A1 and Wx-B1.
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Studium polymorfizmu DNA u tritikale
Svojsíková, Nikola
Recently known varieties of triticale do not meet milling and baking requirements. The reason is a presence of chromosome R in triticale, which decreases viscoelastic qualities of dough, and a presence of secalins, which decreases technological qualities of grain. Bachelor thesis devotes to detection of high-molecular-weight glutenin subunits (HMW-GS) and low-molecular-weight glutenin subunits (LMW-GS). In total there were 14 genotypes of triticale analyzed in the thesis. DNA markers based on polymerase chain reaction (PCR) were used to identify allelic combinations in loci Glu-A1, Glu-B1 and Glu-D1, which encode high-molecular-weight glutenin subunits, and secalin locus. Alleles Glu-A1a, Glu-A1b, Glu-A1c were detected in locus Glu-A1. Four allelic combinations of glutenin subunits Bx7+By18*, Bx7*+By8, Bx7+By8*, Bx7+By8 were detected in locus Glu-B1. Allele Glu-D1d was detected at two breeding lines. Sec-1 was detected at 12 breeding lines. DNA markers were used to discover allelic composition of locus Glu-A3, which encodes low-molecular-weight glutenin subunits. Alleles Glu-A3a, Glu-A3d and Glu-A3f were not detected at analyzed breeding lines at all.
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