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Biological activity of plant metabolites XI. Alkaloids of Corydalis cava (L.) Schweigg & Körte (Fumariaceae) and their influence on acetylcholinesterase
Sekula, Marek ; Opletal, Lubomír (advisor) ; Cahlíková, Lucie (referee)
Sekula, M.: Biological activity of plant metabolites XI.; Alkaloids of Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) and their activity on acetylcholinesterase. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2009, 61 p. Within the screening of plants that contains alkaloids inhibiting the aktivity of the human erythtocytic acetylcholinesterase and human serum butyrylcholinesterase Turkey Corn (Corydalis cava (L.) Schweigg. & Körte, Fumariaceae) was studied. 11,3 kg of the dry roots served for the isolation of alkaloids. Extrakt "B-chloroform" wich contain mixture of terciary alkaloids bad diethylether-insoluble were obtained from the purified ethanolic extract. The task was to separate mixture of alkaloids from extrakt "B-chloroform". Using both column and thin layer chromatography sinoacutine as a base was isolated. The identity of the isolated alkaloid as determined by means of comparing its physico-chemical characteristics with the published data. The isolated sinoacutine inhibited the human erythrocytic acetylcholinesterase and human serum butyrylcholinesterase with IC50 for AChE (1,510 × 10-3 M) and with IC50 for BuChE (1,806 × 10-3 M). Comparing its biological aktivity with that of...
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Biological Active Plant Metabolites I. Alkaloids of Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) and Screening of Their Biological Properties
Sekula, Marek ; Cahlíková, Lucie (advisor) ; Jahodář, Luděk (referee)
Sekula, M.: Biological Active Plant Metabolites I. Alkaloids of Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) and Screening of Their Biological Properties. Rigorous thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2010, 65 p. Within the screening of plants that contains alkaloids inhibiting the activity of the human erythtocytic acetylcholinesterase and human serum butyrylcholinesterase Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) was studied. This work connect to my diploma thesis (2009). The task was to separate mixture of alkaloids from extract "B-chloroform" subfraction F1. This subfraction was prepared within the frame of diploma thesis. From this mixture, using preparative TLC, were isolated two compounds F1-1A and F1-1C in the form of free bases. On the basis of MS, NMR and IR spektroskopy the isolated alkaloid F1-1A was identified as 3-(1-(6,7- dimethoxy-3,4-dihydroisoquinolin-1-yl)ethyl)-2,6-dimethoxy-phenol. It is the first isolation and description of this compound. Structural analysis of compound F1-1C is continuing. The isolated compound F1-1A inhibited the human erythrocyte acetylcholinesterase and human blood serum butyrylcholinesterase with IC50 for AChE (127,6×10-6 M) and with...
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Biological Active Plant Metabolites I. Alkaloids of Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) and Screening of Their Biological Properties
Sekula, Marek ; Jahodář, Luděk (referee) ; Cahlíková, Lucie (advisor)
Sekula, M.: Biological Active Plant Metabolites I. Alkaloids of Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) and Screening of Their Biological Properties. Rigorous thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2010, 65 p. Within the screening of plants that contains alkaloids inhibiting the activity of the human erythtocytic acetylcholinesterase and human serum butyrylcholinesterase Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) was studied. This work connect to my diploma thesis (2009). The task was to separate mixture of alkaloids from extract "B-chloroform" subfraction F1. This subfraction was prepared within the frame of diploma thesis. From this mixture, using preparative TLC, were isolated two compounds F1-1A and F1-1C in the form of free bases. On the basis of MS, NMR and IR spektroskopy the isolated alkaloid F1-1A was identified as 3-(1-(6,7- dimethoxy-3,4-dihydroisoquinolin-1-yl)ethyl)-2,6-dimethoxy-phenol. It is the first isolation and description of this compound. Structural analysis of compound F1-1C is continuing. The isolated compound F1-1A inhibited the human erythrocyte acetylcholinesterase and human blood serum butyrylcholinesterase with IC50 for AChE (127,6×10-6 M) and with...
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Biological activity of plant metabolites XI. Alkaloids of Corydalis cava (L.) Schweigg & Körte (Fumariaceae) and their influence on acetylcholinesterase
Sekula, Marek ; Cahlíková, Lucie (referee) ; Opletal, Lubomír (advisor)
Sekula, M.: Biological activity of plant metabolites XI.; Alkaloids of Corydalis cava (L.) Schweigg. & Körte (Fumariaceae) and their activity on acetylcholinesterase. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2009, 61 p. Within the screening of plants that contains alkaloids inhibiting the aktivity of the human erythtocytic acetylcholinesterase and human serum butyrylcholinesterase Turkey Corn (Corydalis cava (L.) Schweigg. & Körte, Fumariaceae) was studied. 11,3 kg of the dry roots served for the isolation of alkaloids. Extrakt "B-chloroform" wich contain mixture of terciary alkaloids bad diethylether-insoluble were obtained from the purified ethanolic extract. The task was to separate mixture of alkaloids from extrakt "B-chloroform". Using both column and thin layer chromatography sinoacutine as a base was isolated. The identity of the isolated alkaloid as determined by means of comparing its physico-chemical characteristics with the published data. The isolated sinoacutine inhibited the human erythrocytic acetylcholinesterase and human serum butyrylcholinesterase with IC50 for AChE (1,510 × 10-3 M) and with IC50 for BuChE (1,806 × 10-3 M). Comparing its biological aktivity with that of...
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