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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Selected Awarded Works for Children and Youth from the Albatros Publishing House and their Application in Leisure Education for Reading
 SPĚVÁKOVÁ, Daniela
The bachelor's thesis is focused on leisure literary educational activities in the lessons of reading clubs with the aim of preparing worksheets for these lessons. Individual lessons are focused on selected award-winning works from the Albatros publishing house. The works were selected on the basis of their suitability according to the age of the participants, namely for the primary and lower secondary schools. The work briefly describes the history of the Albatros publishing house, the individual awards that are given to the works and a list of the awarded works. The next chapter contains analyses of individual works that were used in the thesis. The analyses are followed by a chapter listing the methods used. In the following chapter, which is already a practical analysis of lectures, lessons of reading clubs for the primary and lower secondary school took place with the help of prepared worksheets. Their overall evaluation is at the end of this chapter.
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The PCR method optimization for analysis of the gene PAI-1 polymorphism
SPĚVÁKOVÁ, Daniela
The plasminogen activator inhibitor (PAI-1) is an important biomarker of fibrinolysis. This glycoprotein acts as a principal inhibitor of plasminogen activators and its high plasma level is associated with various types of diseases, such as cardiovascular diseases or malignant tumors. The PAI-1 gene is located on chromosome 7 in region 7q22.1. In 1993 4G/5G polymorphism was discovered in the gene promoter. The 4G allele increases PAI-1 gene expression which results in a higher plasma level of this inhibitor. That's why this polymorphism has become the subject of many studies. The main aim of my bachelor thesis was to optimize the ARMS-PCR method for analysis of the gene PAI-1 4G/5G polymorphism. Other goals were to write a specialized research on this subject and to learn the techniques used in the genetic laboratory (DNA isolation, DNA concentration measurement, Real-time PCR). In the theoretical part I describe the PAI-1 gene and its product. I deal mainly with the role of this inhibitor in the mechanism of fibrinolysis and its influence on various diseases. Furthermore, I showed an overview of methodological approaches used for genetic analysis of 4G/5G polymorphism in PAI-1 gene. In the practical part there are presented procedures of optimization of the ARMS PCR method and the results of the genotypes of the examined group obtained by the Real-time PCR method, when a commercial kit was used. The optimization of the ARMS-PCR method was not successful.
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