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Influence of enzymatic activity induced by copper and cadmium in aquatic plant Myriophyllum alterniflorum DC.
Mikšová, Martina ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
This thesis concerns with the effects of heavy metals on plants and is a part of a broader research on biomarkers of oxidative stress in aquatic plants in the Department of Pharmaceutical Botany of Faculty of Pharmacy in the University of Limoges. Experimental organism was an aquatic plant Myriophyllum alterniflorum DC (alternate water milfoil) that was collected in the upper stream of the river Vienna. Samples were cultivated in the increasing concentrations of copper and cadmium (0mM, 0,25mM, 0,5mM, 0,75mM) for 1, 3 and 6 days. On account of time and chemical products insufficiency only several analyses of plants treated with copper were carried out. Catalase is one of the enzymes integrated in the antioxidative defence system. It decomposes H2O2 into water. During the time catalase activity gradually increased up to 0,5mM. 0,75mM showed the maximum after the third day. When comparing different copper concentrations after the same cultivation period the highest activity was observed in 0,25mM in the first day and in 0,50mM the third and sixth day. Myriophyllum alterniflorum DC as other aquatic plants exhibits the heavy metal accumulation ability increasing in time and higher copper concentrations. On the contrary, decreasing time and concentration dependence was observable in measurements of...
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Influence of enzymatic activity induced by copper and cadmium in aquatic plant Myriophyllum alterniflorum DC.
Mikšová, Martina ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
This thesis concerns with the effects of heavy metals on plants and is a part of a broader research on biomarkers of oxidative stress in aquatic plants in the Department of Pharmaceutical Botany of Faculty of Pharmacy in the University of Limoges. Experimental organism was an aquatic plant Myriophyllum alterniflorum DC (alternate water milfoil) that was collected in the upper stream of the river Vienna. Samples were cultivated in the increasing concentrations of copper and cadmium (0mM, 0,25mM, 0,5mM, 0,75mM) for 1, 3 and 6 days. On account of time and chemical products insufficiency only several analyses of plants treated with copper were carried out. Catalase is one of the enzymes integrated in the antioxidative defence system. It decomposes H2O2 into water. During the time catalase activity gradually increased up to 0,5mM. 0,75mM showed the maximum after the third day. When comparing different copper concentrations after the same cultivation period the highest activity was observed in 0,25mM in the first day and in 0,50mM the third and sixth day. Myriophyllum alterniflorum DC as other aquatic plants exhibits the heavy metal accumulation ability increasing in time and higher copper concentrations. On the contrary, decreasing time and concentration dependence was observable in measurements of...
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